Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

Satouh, Yuhkoh; Inoue, Naokazu; Ikawa, Masahito et al.

Journal of Cell Science, 2012, 125(21), 4985-4990

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Identifier to cite or link to this item: https://hdl.handle.net/11094/78608

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Title
Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
Creator
NRID
1000040545571 1000040545571
CreatorName
Satouh, Yuhkoh
NRID
1000050379096 1000050379096
CreatorName
Inoue, Naokazu
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
CreatorName
Ikawa, Masahito
NRID
1000030089875 1000030089875
CreatorName
Okabe, Masaru
Subject
Acrosome reaction
IZUMO1
Live-cell imaging
Membrane fusion
Protein trafficking
Sperm-egg fusion
Abstract
Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent protein tag. IZUMO1 showed a tendency to localize in the equatorial segment of the sperm surface after the acrosome reaction. This region is considered to initiate fusion with the oolemma. The moment of sperm-egg fusion and the dynamic movements of proteins during fusion were also imaged live. Translocation of IZUMO1 during the fertilization process was clarified, and a fundamental mechanism in mammalian fertilization is postulated.
Publisher
Company of Biologists Ltd
Source Title
Journal of Cell Science
Volume (Issue)
125(21)
Page
4985-4990
Date of Issued
2012-11-01
Language
English
Handle URL
https://hdl.handle.net/11094/78608
PISSN
00219533
eISSN
14779137
NCID
AA00694823
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1242/jcs.100867
PMID
22946049
Access Rights
open access
CopyRight
© 2012. Published by The Company of Biologists Ltd.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article