Calsperin is a testis-specific chaperone required for sperm fertility

Ikawa, Masahito; Tokuhiro, Keizo; Yamaguchi, Ryo; Benham, Adam M.; Tamura, Taku; Wada, Ikuo; Satouh, Yuhkoh; Inoue, Naokazu; Okabe, Masaru

doi:https://doi.org/10.1074/jbc.M110.140152

Calsperin is a testis-specific chaperone required for sperm fertility

Ikawa, Masahito; Tokuhiro, Keizo; Yamaguchi, Ryo et al.

Journal of Biological Chemistry, 2011, 286(7), 5639-5646

Number of Access:893（2025-08-22 01:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78613

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
JBiolChem_286_07_5639	pdf	None	2.37 MB	143	2021.02.08

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Calsperin is a testis-specific chaperone required for sperm fertility
Creator	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	CreatorName Tokuhiro, Keizo
	CreatorName Yamaguchi, Ryo
	CreatorName Benham, Adam M.
	CreatorName Tamura, Taku
	NRID 1000040182969 1000040182969 CreatorName Wada, Ikuo
	NRID 1000040545571 1000040545571 CreatorName Satouh, Yuhkoh
	NRID 1000050379096 1000050379096 CreatorName Inoue, Naokazu
	NRID 1000030089875 1000030089875 CreatorName Okabe, Masaru
Description	Masahito Ikawa, Keizo Tokuhiro, Ryo Yamaguchi, Adam M. Benham, Taku Tamura, Ikuo Wada, Yuhkoh Satouh, Naokazu Inoue, Masaru Okabe, Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility, Journal of Biological Chemistry, Volume 286, Issue 7, 2011, Pages 5639-5646, ISSN 0021-9258, https://doi.org/10.1074/jbc.M110.140152.
Abstract	Calnexin (CANX) and calreticulin (CALR) are homologous lectin chaperones located in the endoplasmic reticulum and cooperate to mediate nascent glycoprotein folding. In the testis, calmegin (CLGN) and calsperin (CALR3) are expressed as germ cell-specific counterparts of CANX and CALR, respectively. Here, we show that Calr3-/- males produced apparently normal sperm but were infertile because of defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida. Whereas CLGN was required for ADAM1A/ADAM2 dimerization and subsequent maturation of ADAM3, a sperm membrane protein required for fertilization, we show that CALR3 is a lectin-deficient chaperone directly required for ADAM3 maturation. Our results establish the client specificity of CALR3 and demonstrate that the germ cell-specific CALR-like endoplasmic reticulum chaperones have contrasting functions in the development of male fertility. The identification and understanding of the maturation mechanisms of key sperm proteins will pave the way toward novel approaches for both contraception and treatment of unexplained male infertility.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	286(7)
Page	5639-5646
Date of Issued	2011-02-18
Language	English
Handle URL	https://hdl.handle.net/11094/78613
PISSN	00219258
eISSN	1083351X
NCID	AA1202441X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M110.140152
Relation.isIdenticalTo	PMID 21131354
Access Rights	open access
CopyRight	© 2011 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2011, 286(7), p. 5639-5646
DC.title	Calsperin is a testis-specific chaperone required for sperm fertility
DC.creator	Ikawa, Masahito
	Tokuhiro, Keizo
	Yamaguchi, Ryo
	Benham, Adam M.
	Tamura, Taku
	Wada, Ikuo
	Satouh, Yuhkoh
	Inoue, Naokazu
	Okabe, Masaru
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-02-18
DC.identifier	https://doi.org/10.1074/jbc.M110.140152
DC.description	Masahito Ikawa, Keizo Tokuhiro, Ryo Yamaguchi, Adam M. Benham, Taku Tamura, Ikuo Wada, Yuhkoh Satouh, Naokazu Inoue, Masaru Okabe, Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility, Journal of Biological Chemistry, Volume 286, Issue 7, 2011, Pages 5639-5646, ISSN 0021-9258, https://doi.org/10.1074/jbc.M110.140152.
DCTERMS.abstract	Calnexin (CANX) and calreticulin (CALR) are homologous lectin chaperones located in the endoplasmic reticulum and cooperate to mediate nascent glycoprotein folding. In the testis, calmegin (CLGN) and calsperin (CALR3) are expressed as germ cell-specific counterparts of CANX and CALR, respectively. Here, we show that Calr3-/- males produced apparently normal sperm but were infertile because of defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida. Whereas CLGN was required for ADAM1A/ADAM2 dimerization and subsequent maturation of ADAM3, a sperm membrane protein required for fertilization, we show that CALR3 is a lectin-deficient chaperone directly required for ADAM3 maturation. Our results establish the client specificity of CALR3 and demonstrate that the germ cell-specific CALR-like endoplasmic reticulum chaperones have contrasting functions in the development of male fertility. The identification and understanding of the maturation mechanisms of key sperm proteins will pave the way toward novel approaches for both contraception and treatment of unexplained male infertility.
DC.rights	© 2011 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78613
citation_title	Calsperin is a testis-specific chaperone required for sperm fertility
citation_author	Ikawa, Masahito
	Tokuhiro, Keizo
	Yamaguchi, Ryo
	Benham, Adam M.
	Tamura, Taku
	Wada, Ikuo
	Satouh, Yuhkoh
	Inoue, Naokazu
	Okabe, Masaru
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2011-02-18
citation_journal_title	Journal of Biological Chemistry
citation_volume	286
citation_issue	7
citation_firstpage	5639
citation_lastpage	5646
citation_doi	https://doi.org/10.1074/jbc.M110.140152
citation_pmid	21131354
citation_public_url	https://hdl.handle.net/11094/78613

Calsperin is a testis-specific chaperone required for sperm fertility

Link to primary information

Item Information

Show details