Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

Fujihara, Yoshitaka; Murakami, Masao; Inoue, Naokazu; Satouh, Yuhkoh; Kaseda, Kazuhiro; Ikawa, Masahito; Okabe, Masaru

doi:https://doi.org/10.1242/jcs.067363

Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

Fujihara, Yoshitaka; Murakami, Masao; Inoue, Naokazu et al.

Journal of Cell Science, 2010, 123(9), 1531-1536

Number of Access:717（2025-08-22 01:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/78617

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Title	Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse
Creator	NRID 1000070578848 1000070578848 CreatorName Fujihara, Yoshitaka
	NRID 1000050210018 1000050210018 CreatorName Murakami, Masao
	NRID 1000050379096 1000050379096 CreatorName Inoue, Naokazu
	NRID 1000040545571 1000040545571 CreatorName Satouh, Yuhkoh
	CreatorName Kaseda, Kazuhiro
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 CreatorName Ikawa, Masahito
	NRID 1000030089875 1000030089875 CreatorName Okabe, Masaru
Subject	Equatorial segment
	Fertilization
	Knockout mouse
	Sperm-egg fusion
Abstract	Mammalian fertilization is a multistep process that culminates in the fusion of the sperm and egg plasma membrane. It is widely accepted that the equatorial segment of the acrosome-reacted sperm is important in initiating fusion with the egg plasma membrane during fertilization. There are various proteins known to be distributed only in the equatorial segment of sperm. The role of these proteins must be clarified to understand the membrane fusion process. We produced a mouse line that lacked SPESP1 (sperm equatorial segment protein 1) and analyzed the fertilizing ability of the sperm. The average number of pups that were fathered by Spesp1+/- and Spesp1-/- males was significantly lower than that of wild-type fathers. In these mouse lines, fewer sperm were found to migrate into oviducts and fewer eggs were fertilized. The Spesp1+/- and Spesp1-/- sperm showed a lower fusing ability compared with the wildtype sperm. The disruption of Spesp1 was shown to cause an aberrant distribution of various sperm proteins. Moreover, scanning electron microscopy revealed that the membrane in the equatorial segment area, which usually forms an acrosomal sheath, disappears after acrosome reaction in Spesp1-deficient mice. It was demonstrated that SPESP1 is necessary to produce the fully 'fusion competent' sperm.
Publisher	Company of Biologists Ltd
Source Title	Journal of Cell Science
Volume (Issue)	123(9)
Page	1531-1536
Date of Issued	2010-05-01
Language	English
Handle URL	https://hdl.handle.net/11094/78617
PISSN	00219533
eISSN	14779137
NCID	AA00694823
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1242/jcs.067363
Relation.isIdenticalTo	PMID 20375058
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Journal of Cell Science. 2010, 123(9), p. 1531-1536
DC.title	Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse
DC.creator	Fujihara, Yoshitaka
	Murakami, Masao
	Inoue, Naokazu
	Satouh, Yuhkoh
	Kaseda, Kazuhiro
	Ikawa, Masahito
	Okabe, Masaru
DC.publisher	Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-05-01
DC.identifier	https://doi.org/10.1242/jcs.067363
DC.subject	Equatorial segment
	Fertilization
	Knockout mouse
	Sperm-egg fusion
DCTERMS.abstract	Mammalian fertilization is a multistep process that culminates in the fusion of the sperm and egg plasma membrane. It is widely accepted that the equatorial segment of the acrosome-reacted sperm is important in initiating fusion with the egg plasma membrane during fertilization. There are various proteins known to be distributed only in the equatorial segment of sperm. The role of these proteins must be clarified to understand the membrane fusion process. We produced a mouse line that lacked SPESP1 (sperm equatorial segment protein 1) and analyzed the fertilizing ability of the sperm. The average number of pups that were fathered by Spesp1+/- and Spesp1-/- males was significantly lower than that of wild-type fathers. In these mouse lines, fewer sperm were found to migrate into oviducts and fewer eggs were fertilized. The Spesp1+/- and Spesp1-/- sperm showed a lower fusing ability compared with the wildtype sperm. The disruption of Spesp1 was shown to cause an aberrant distribution of various sperm proteins. Moreover, scanning electron microscopy revealed that the membrane in the equatorial segment area, which usually forms an acrosomal sheath, disappears after acrosome reaction in Spesp1-deficient mice. It was demonstrated that SPESP1 is necessary to produce the fully 'fusion competent' sperm.
DC.format	78617
citation_title	Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse
citation_author	Fujihara, Yoshitaka
	Murakami, Masao
	Inoue, Naokazu
	Satouh, Yuhkoh
	Kaseda, Kazuhiro
	Ikawa, Masahito
	Okabe, Masaru
citation_publisher	Company of Biologists Ltd
citation_language	English
citation_date	2010-05-01
citation_journal_title	Journal of Cell Science
citation_volume	123
citation_issue	9
citation_firstpage	1531
citation_lastpage	1536
citation_doi	https://doi.org/10.1242/jcs.067363
citation_pmid	20375058
citation_public_url	https://hdl.handle.net/11094/78617

Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

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