In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation

Watanabe, Wataru; Matsunaga, Sachihiro; Higashi, Tsunehito et al.

Journal of Biomedical Optics, 2008, 13(3), 031213

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Identifier to cite or link to this item: https://hdl.handle.net/11094/78973

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Title
In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation
Creator
NRID
1000090314377 1000090314377
CreatorName
Watanabe, Wataru
NRID
1000040323448 1000040323448
CreatorName
Matsunaga, Sachihiro
NRID
1000090453018 1000090453018
CreatorName
Higashi, Tsunehito
NRID
1000000311770 1000000311770
researchmap permalink
read0068600 read0068600
CreatorName
Fukui, Kiichi
NRID
1000080113520 1000080113520
CreatorName
Itoh, Kazuyoshi
Subject
ablation
femtosecond laser
fluorescence recovery after photobleaching
nanosurgery
organelles
photoconvertible fluorescent protein
two-photon microscopy
Description
Wataru Watanabe, Sachhiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, and Kazuyoshi Itoh "In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation," Journal of Biomedical Optics 13(3), 031213 (1 May 2008). https://doi.org/10.1117/1.2939401
Abstract
Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein. © 2008 Society of Photo-Optical Instrumentation Engineers.
Publisher
SPIE
Source Title
Journal of Biomedical Optics
Volume (Issue)
13(3)
Page
031213
Date of Issued
2008-05-01
Language
English
Handle URL
https://hdl.handle.net/11094/78973
PISSN
10833668
eISSN
15602281
NCID
AA11237889
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1117/1.2939401
PMID
18601537
Access Rights
open access
CopyRight
Copyright 2008 Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article