Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis

Takata, Hideaki; Madung, Marliza; Katoh, Kaoru et al.

PLoS ONE, 2018, 13(12), e0209614

Number of Access:465（2025-08-14 18:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79022

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Title
Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis
Creator
NRID
1000020455207 1000020455207
CreatorName
Takata, Hideaki
CreatorName
Madung, Marliza
NRID
1000080356782 1000080356782
CreatorName
Katoh, Kaoru
NRID
1000000311770 1000000311770
researchmap permalink
read0068600 read0068600
CreatorName
Fukui, Kiichi
Description
Takata H, Madung M, Katoh K, Fukui K (2018) Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis. PLOS ONE 13(12): e0209614. https://doi.org/10.1371/journal.pone.0209614.
Abstract
Chromosome organization during cell division is achieved through the timely association of proteins with chromatin and is regulated by protein phosphorylation. Kinesin family member 4A (KIF4A) plays an important role in the chromosome organization through the formation of the chromosome scaffold structure. However, the relationship between the function of KIF4A and its phosphorylation remains unclear. Here, we demonstrate that Cdk1-depen-dent phosphorylation of KIF4A at S1186 is required for chromosome binding and chromosome scaffold formation. The KIF4A mutant, which is not phosphorylated at S1186, was found to localize to the nucleus during interphase but did not accumulate in the chromosome scaffold after nuclear envelope breakdown. In addition, defects in KIF4A phosphorylation were found to disrupt the interaction of KIF4A with the condensin I complex. As a result, the morphology of the chromosomes was observed to be laterally decondensed, without condensin I in the chromosome scaffold. Additionally, a defect in chromosome segregation, chromosome bridge formation, was often observed. Although both KIF4A and condensin I disappeared from the chromosomes, the chromosomal localization of condensin II was not affected. Collectively, our novel results revealed that Cdk1-dependent KIF4A phosphorylation at S1186 is a trigger for chromosomal organization during early mitosis.
Publisher
Public Library of Science
Source Title
PLoS ONE
Volume (Issue)
13(12)
Page
e0209614
Date of Issued
2018-12-21
Language
English
Handle URL
https://hdl.handle.net/11094/79022
eISSN
19326203
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1371/journal.pone.0209614
PMID
30576375
Access Rights
open access
CopyRight
© 2018 Takata et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article