Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Phengchat, Rinyaporn; Takata, Hideaki; Uchiyama, Susumu et al.

Scientific Reports, 2017, 7(1), 7335

Number of Access:700（2025-08-14 07:54 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79024

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Title
Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
Creator
CreatorName
Phengchat, Rinyaporn
NRID
1000020455207 1000020455207
CreatorName
Takata, Hideaki
NRID
1000090335381 1000090335381
ORCID
0000-0002-5181-179X 0000-0002-5181-179X
researchmap permalink
read0090387 read0090387
Scopus Author ID
35429790100 35429790100
CreatorName
Uchiyama, Susumu
NRID
1000000311770 1000000311770
researchmap permalink
read0068600 read0068600
CreatorName
Fukui, Kiichi
Description
Phengchat, R., Takata, H., Uchiyama, S. et al. Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore. Sci Rep 7, 7335 (2017). https://doi.org/10.1038/s41598-017-07777-6.
Abstract
The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca²⁺ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the spindle organisation in mitotic cells remains controversial. Here, we investigated the role of Ca²⁺ on kinetochore fibres in living cells. We found that depletion of Ca²⁺ during mitosis reduced kinetochore fibre stability. Reduction of kinetochore fibre stability was not due to direct inhibition of microtubule polymerisation by Ca2⁺- depletion but due to elimination of one dynamic component of kinetochore, CENP-F from the kinetochore. This compromised the attachment of kinetochore fibres to the kinetochore which possibly causes mitotic defects induced by the depletion of Ca²⁺.
Publisher
Nature Research
Source Title
Scientific Reports
Volume (Issue)
7(1)
Page
7335
Date of Issued
2017-08-04
Language
English
Handle URL
https://hdl.handle.net/11094/79024
eISSN
20452322
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1038/s41598-017-07777-6
PMID
28779172
Access Rights
open access
CopyRight
© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article