Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition

Uchiyama, Susumu; Kawahara, Kazuki; Hosokawa, Yuki; Fukakusa, Shunsuke; Oki, Hiroya; Nakamura, Shota; Kojima, Yukiko; Noda, Masanori; Takino, Rie; Miyahara, Yuya; Maruno, Takahiro; Kobayashi, Yuji; Ohkubo, Tadayasu; Fukui, Kiichi

doi:https://doi.org/10.1074/jbc.M115.670794

Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition

Uchiyama, Susumu; Kawahara, Kazuki; Hosokawa, Yuki et al.

Journal of Biological Chemistry, 2015, 290(49), 29461-29477

Number of Access:1,860（2025-08-14 04:16 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79029

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
JBiolChem_290_49_29461	pdf	None	4.71 MB	128	2021.03.16

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition
Creator	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000060585058 1000060585058 researchmap permalink 051619 051619 Scopus Author ID 9740512500 9740512500 CreatorName Kawahara, Kazuki
	CreatorName Hosokawa, Yuki
	CreatorName Fukakusa, Shunsuke
	NRID 1000030845285 1000030845285 researchmap permalink hiroya168 hiroya168 CreatorName Oki, Hiroya
	NRID 1000090432434 1000090432434 ORCID 0000-0003-2058-5942 0000-0003-2058-5942 researchmap permalink nkmrshota nkmrshota Scopus Author ID 35315778200 35315778200 CreatorName Nakamura, Shota
	CreatorName Kojima, Yukiko
	CreatorName Noda, Masanori
	CreatorName Takino, Rie
	CreatorName Miyahara, Yuya
	NRID 1000060648351 1000060648351 CreatorName Maruno, Takahiro
	NRID 1000020127228 1000020127228 CreatorName Kobayashi, Yuji
	NRID 1000090272997 1000090272997 researchmap permalink read0191274 read0191274 Scopus Author ID 7201618682 7201618682 CreatorName Ohkubo, Tadayasu
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Description	Susumu Uchiyama, Kazuki Kawahara, Yuki Hosokawa, Shunsuke Fukakusa, Hiroya Oki, Shota Nakamura, Yukiko Kojima, Masanori Noda, Rie Takino, Yuya Miyahara, Takahiro Maruno, Yuji Kobayashi, Tadayasu Ohkubo, Kiichi Fukui. Structural Basis for Dimer Formation of Human Condensin Structural Maintenance of Chromosome Proteins and Its Implications for Single-stranded DNA Recognition. Journal of Biological Chemistry, Volume 290, Issue 49, 2015, Pages 29461-29477. https://doi.org/10.1074/jbc.M115.670794.
Abstract	Eukaryotic structural maintenance of chromosome proteins (SMC) are major components of cohesin and condensins that regulate chromosome structure and dynamics during cell cycle. We here determine the crystal structure of human condensin SMC hinge heterodimer with ∼30 residues of coiled coils. The structure, in conjunction with the hydrogen exchange mass spectrometry analyses, revealed the structural basis for the specific heterodimer formation of eukaryotic SMC and that the coiled coils from two different hinges protrude in the same direction, providing a unique binding surface conducive for binding to single-stranded DNA. The characteristic hydrogen exchange profiles of peptides constituted regions especially across the hinge-hinge dimerization interface, further suggesting the structural alterations upon single-stranded DNA binding and the presence of a half-opened state of hinge heterodimer. This structural change potentially relates to the DNA loading mechanism of SMC, in which the hinge domain functions as an entrance gate as previously proposed for cohesin. Our results, however, indicated that this is not the case for condensins based on the fact that the coiled coils are still interacting with each other, even when DNA binding induces structural changes in the hinge region, suggesting the functional differences of SMC hinge domain between condensins and cohesin in DNA recognition.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	290(49)
Page	29461-29477
Date of Issued	2015-12-04
Language	English
Handle URL	https://hdl.handle.net/11094/79029
PISSN	00219258
eISSN	1083351X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M115.670794
Relation.isIdenticalTo	PMID 26491021
Access Rights	open access
CopyRight	© 2015 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2015, 290(49), p. 29461-29477
DC.title	Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition
DC.creator	Uchiyama, Susumu
	Kawahara, Kazuki
	Hosokawa, Yuki
	Fukakusa, Shunsuke
	Oki, Hiroya
	Nakamura, Shota
	Kojima, Yukiko
	Noda, Masanori
	Takino, Rie
	Miyahara, Yuya
	Maruno, Takahiro
	Kobayashi, Yuji
	Ohkubo, Tadayasu
	Fukui, Kiichi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2015-12-04
DC.identifier	https://doi.org/10.1074/jbc.M115.670794
DC.description	Susumu Uchiyama, Kazuki Kawahara, Yuki Hosokawa, Shunsuke Fukakusa, Hiroya Oki, Shota Nakamura, Yukiko Kojima, Masanori Noda, Rie Takino, Yuya Miyahara, Takahiro Maruno, Yuji Kobayashi, Tadayasu Ohkubo, Kiichi Fukui. Structural Basis for Dimer Formation of Human Condensin Structural Maintenance of Chromosome Proteins and Its Implications for Single-stranded DNA Recognition. Journal of Biological Chemistry, Volume 290, Issue 49, 2015, Pages 29461-29477. https://doi.org/10.1074/jbc.M115.670794.
DCTERMS.abstract	Eukaryotic structural maintenance of chromosome proteins (SMC) are major components of cohesin and condensins that regulate chromosome structure and dynamics during cell cycle. We here determine the crystal structure of human condensin SMC hinge heterodimer with ∼30 residues of coiled coils. The structure, in conjunction with the hydrogen exchange mass spectrometry analyses, revealed the structural basis for the specific heterodimer formation of eukaryotic SMC and that the coiled coils from two different hinges protrude in the same direction, providing a unique binding surface conducive for binding to single-stranded DNA. The characteristic hydrogen exchange profiles of peptides constituted regions especially across the hinge-hinge dimerization interface, further suggesting the structural alterations upon single-stranded DNA binding and the presence of a half-opened state of hinge heterodimer. This structural change potentially relates to the DNA loading mechanism of SMC, in which the hinge domain functions as an entrance gate as previously proposed for cohesin. Our results, however, indicated that this is not the case for condensins based on the fact that the coiled coils are still interacting with each other, even when DNA binding induces structural changes in the hinge region, suggesting the functional differences of SMC hinge domain between condensins and cohesin in DNA recognition.
DC.rights	© 2015 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	79029
citation_title	Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition
citation_author	Uchiyama, Susumu
	Kawahara, Kazuki
	Hosokawa, Yuki
	Fukakusa, Shunsuke
	Oki, Hiroya
	Nakamura, Shota
	Kojima, Yukiko
	Noda, Masanori
	Takino, Rie
	Miyahara, Yuya
	Maruno, Takahiro
	Kobayashi, Yuji
	Ohkubo, Tadayasu
	Fukui, Kiichi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2015-12-04
citation_journal_title	Journal of Biological Chemistry
citation_volume	290
citation_issue	49
citation_firstpage	29461
citation_lastpage	29477
citation_doi	https://doi.org/10.1074/jbc.M115.670794
citation_pmid	26491021
citation_public_url	https://hdl.handle.net/11094/79029

Structural basis for dimer formation of human condensin structural maintenance of chromosome proteins and its implications for single-stranded DNA recognition

Link to primary information

Item Information

Show details