RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion

Matsunaga, Sachihiro; Takata, Hideaki; Morimoto, Akihiro; Hayashihara, Kayoko; Higashi, Tsunehito; Akatsuchi, Kouhei; Mizusawa, Eri; Yamakawa, Mariko; Ashida, Mamoru; Matsunaga, Tomoko M.; Azuma, Takachika; Uchiyama, Susumu; Fukui, Kiichi

doi:https://doi.org/10.1016/j.celrep.2012.02.005

RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion

Matsunaga, Sachihiro; Takata, Hideaki; Morimoto, Akihiro et al.

Cell Reports, 2012, 1(4), 299-308

Number of Access:716（2025-06-25 13:56 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79037

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Title	RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion
Creator	NRID 1000040323448 1000040323448 CreatorName Matsunaga, Sachihiro
	NRID 1000020455207 1000020455207 CreatorName Takata, Hideaki
	NRID 1000000142307 1000000142307 CreatorName Morimoto, Akihiro
	NRID 1000000624389 1000000624389 CreatorName Hayashihara, Kayoko
	NRID 1000090453018 1000090453018 CreatorName Higashi, Tsunehito
	CreatorName Akatsuchi, Kouhei
	CreatorName Mizusawa, Eri
	CreatorName Yamakawa, Mariko
	CreatorName Ashida, Mamoru
	CreatorName Matsunaga, Tomoko M.
	NRID 1000000028234 1000000028234 CreatorName Azuma, Takachika
	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Description	Sachihiro Matsunaga, Hideaki Takata, Akihiro Morimoto, Kayoko Hayashihara, Tsunehito Higashi, Kouhei Akatsuchi, Eri Mizusawa, Mariko Yamakawa, Mamoru Ashida, Tomoko M. Matsunaga, Takachika Azuma, Susumu Uchiyama, Kiichi Fukui. RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion. Cell Reports, Volume 1, Issue 4, 2012, Pages 299-308. https://doi.org/10.1016/j.celrep.2012.02.005.
Abstract	Cohesion is essential for the identification of sister chromatids and for the biorientation of chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on the X chromosome (RBMX) plays an essential role in chromosome morphogenesis through its association with chromatin, but not with RNA. Depletion of RBMX by RNA interference (RNAi) causes the loss of cohesin from the centromeric regions before anaphase, resulting in premature chromatid separation accompanied by delocalization of the shugoshin complex and outer kinetochore proteins. Cohesion defects caused by RBMX depletion can be detected as early as the G2 phase. Moreover, RBMX associates with the cohesin subunits, Scc1 and Smc3, and with the cohesion regulator, Wapl. RBMX is required for cohesion only in the presence of Wapl, suggesting that RBMX is an inhibitor of Wapl. We propose that RBMX is a cohesion regulator that maintains the proper cohesion of sister chromatids.
Publisher	Elsevier
Source Title	Cell Reports
Volume (Issue)	1(4)
Page	299-308
Date of Issued	2012-04-19
Language	English
Handle URL	https://hdl.handle.net/11094/79037
eISSN	22111247
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.celrep.2012.02.005
Relation.isIdenticalTo	PMID 22832223
Access Rights	open access
CopyRight	© 2012 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Cell Reports. 2012, 1(4), p. 299-308
DC.title	RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion
DC.creator	Matsunaga, Sachihiro
	Takata, Hideaki
	Morimoto, Akihiro
	Hayashihara, Kayoko
	Higashi, Tsunehito
	Akatsuchi, Kouhei
	Mizusawa, Eri
	Yamakawa, Mariko
	Ashida, Mamoru
	Matsunaga, Tomoko M.
	Azuma, Takachika
	Uchiyama, Susumu
	Fukui, Kiichi
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2012-04-19
DC.identifier	https://doi.org/10.1016/j.celrep.2012.02.005
DC.description	Sachihiro Matsunaga, Hideaki Takata, Akihiro Morimoto, Kayoko Hayashihara, Tsunehito Higashi, Kouhei Akatsuchi, Eri Mizusawa, Mariko Yamakawa, Mamoru Ashida, Tomoko M. Matsunaga, Takachika Azuma, Susumu Uchiyama, Kiichi Fukui. RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion. Cell Reports, Volume 1, Issue 4, 2012, Pages 299-308. https://doi.org/10.1016/j.celrep.2012.02.005.
DCTERMS.abstract	Cohesion is essential for the identification of sister chromatids and for the biorientation of chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on the X chromosome (RBMX) plays an essential role in chromosome morphogenesis through its association with chromatin, but not with RNA. Depletion of RBMX by RNA interference (RNAi) causes the loss of cohesin from the centromeric regions before anaphase, resulting in premature chromatid separation accompanied by delocalization of the shugoshin complex and outer kinetochore proteins. Cohesion defects caused by RBMX depletion can be detected as early as the G2 phase. Moreover, RBMX associates with the cohesin subunits, Scc1 and Smc3, and with the cohesion regulator, Wapl. RBMX is required for cohesion only in the presence of Wapl, suggesting that RBMX is an inhibitor of Wapl. We propose that RBMX is a cohesion regulator that maintains the proper cohesion of sister chromatids.
DC.rights	© 2012 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
DC.format	79037
citation_title	RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion
citation_author	Matsunaga, Sachihiro
	Takata, Hideaki
	Morimoto, Akihiro
	Hayashihara, Kayoko
	Higashi, Tsunehito
	Akatsuchi, Kouhei
	Mizusawa, Eri
	Yamakawa, Mariko
	Ashida, Mamoru
	Matsunaga, Tomoko M.
	Azuma, Takachika
	Uchiyama, Susumu
	Fukui, Kiichi
citation_publisher	Elsevier
citation_language	English
citation_date	2012-04-19
citation_journal_title	Cell Reports
citation_volume	1
citation_issue	4
citation_firstpage	299
citation_lastpage	308
citation_doi	https://doi.org/10.1016/j.celrep.2012.02.005
citation_pmid	22832223
citation_public_url	https://hdl.handle.net/11094/79037

RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion

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