Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase

Kurihara, Daisuke; Matsunaga, Sachihiro; Omura, Tomohiro et al.

BMC Plant Biology, 2011, 11, 73

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Identifier to cite or link to this item: https://hdl.handle.net/11094/79038

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Title
Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase
Creator
NRID
1000090609439 1000090609439
CreatorName
Kurihara, Daisuke
NRID
1000040323448 1000040323448
CreatorName
Matsunaga, Sachihiro
CreatorName
Omura, Tomohiro
NRID
1000000313205 1000000313205
CreatorName
Higashiyama, Tetsuya
NRID
1000000311770 1000000311770
researchmap permalink
read0068600 read0068600
CreatorName
Fukui, Kiichi
Description
Kurihara, D., Matsunaga, S., Omura, T. et al. Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase. BMC Plant Biol 11, 73 (2011). https://doi.org/10.1186/1471-2229-11-73.
Abstract
Background: Haspin kinases are mitotic kinases that are well-conserved from yeast to human. Human Haspin is a histone H3 Thr3 kinase that has important roles in chromosome cohesion during mitosis. Moreover, phosphorylation of histone H3 at Thr3 by Haspin in fission yeast, Xenopus, and human is required for accumulation of Aurora B on the centromere, and the subsequent activation of Aurora B kinase activity for accurate chromosome alignment and segregation. Although extensive analyses of Haspin have been carried out in yeast and animals, the function of Haspin in organogenesis remains unclear.Results: Here, we identified a Haspin kinase, designated AtHaspin, in Arabidopsis thaliana. The purified AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP-α-tubulin in BY-2 cells showed that AtHaspin-tdTomato localized on chromosomes during prometaphase and metaphase, and around the cell plate during cytokinesis. This localization of AtHaspin overlapped with that of phosphorylated Thr3 and Thr11 of histone H3 in BY-2 cells. AtHaspin-GFP driven by the native promoter was expressed in root meristems, shoot meristems, floral meristems, and throughout the whole embryo at stages of high cell division. Overexpression of a kinase domain mutant of AtHaspin decreased the size of the root meristem, which delayed root growth.Conclusions: Our results indicated that the Haspin kinase is a histone H3 threonine kinase in A. thaliana. AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. The expression and dominant-negative analysis showed that AtHaspin may have a role in mitotic cell division during plant growth. Further analysis of coordinated mechanisms involving Haspin and Aurora kinases will shed new light on the regulation of chromosome segregation in cell division during plant growth and development.
Publisher
BioMed Central
Source Title
BMC Plant Biology
Volume (Issue)
11
Page
73
Date of Issued
2011-04-28
Language
English
Handle URL
https://hdl.handle.net/11094/79038
eISSN
14712229
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1186/1471-2229-11-73
PMID
21527018
Access Rights
open access
CopyRight
© 2011 Kurihara et al; licensee BioMed Central Ltd. This article is licensed under a Creative Commons Attribution 2.0 Generic License.
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Category
学術雑誌論文 Journal Article