The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles

Ma, Nan; Matsunaga, Sachihiro; Morimoto, Akihiro; Sakashita, Gyosuke; Urano, Takeshi; Uchiyama, Susumu; Fukui, Kiichi

doi:https://doi.org/10.1242/jcs.063347

The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles

Ma, Nan; Matsunaga, Sachihiro; Morimoto, Akihiro et al.

Journal of Cell Science, 2011, 124(3), 394-404

Number of Access:789（2025-08-14 18:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79039

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Title	The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles
Creator	CreatorName Ma, Nan
	NRID 1000040323448 1000040323448 CreatorName Matsunaga, Sachihiro
	NRID 1000000142307 1000000142307 CreatorName Morimoto, Akihiro
	NRID 1000000397457 1000000397457 CreatorName Sakashita, Gyosuke
	NRID 1000070293701 1000070293701 CreatorName Urano, Takeshi
	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Subject	Aurora-A
	Kinetochore-microtubule attachment
	Mitosis
	Nucleolin
	SAF-A
	Spindle assembly
	TPX2
Description	Ma N., Matsunaga S., Morimoto A., et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. Journal of Cell Science, 124, 3, 394. https://doi.org/10.1242/jcs.063347.
Abstract	Segregation of chromosomes during cell division requires correct formation of mitotic spindles. Here, we show that a scaffold attachment factor A (SAF-A), also known as heterogeneous nuclear ribonucleoprotein-U, contributes to the attachment of spindle microtubules (MTs) to kinetochores and spindle organization. During mitosis, SAF-A was localized at the spindles, spindle midzone and cytoplasmic bridge. Depletion of SAF-A by RNA interference induced mitotic delay and defects in chromosome alignment and spindle assembly. We found that SAF-A specifically co-immunoprecipitated with the chromosome peripheral protein nucleolin and the spindle regulators Aurora-A and TPX2, indicating that SAF-A is associated with nucleolin and the Aurora-A-TPX2 complex. SAF-A was colocalized with TPX2 and Aurora-A in spindle poles and MTs. Elimination of TPX2 or Aurora-A from cells abolished the association of SAF-A with the mitotic spindle. Interestingly, SAF-A can bind to MTs and contributes to the targeting of Aurora-A to mitotic spindle MTs. Our finding indicates that SAF-A is a novel spindle regulator that plays an essential role in kinetochore-MT attachment and mitotic spindle organization.
Publisher	The Company of Biologists Ltd
Source Title	Journal of Cell Science
Volume (Issue)	124(3)
Page	394-404
Date of Issued	2011-01-17
Language	English
Handle URL	https://hdl.handle.net/11094/79039
PISSN	00219533
eISSN	14779137
NCID	AA00694823
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1242/jcs.063347
Relation.isIdenticalTo	PMID 21242313
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Cell Science. 2011, 124(3), p. 394-404
DC.title	The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles
DC.creator	Ma, Nan
	Matsunaga, Sachihiro
	Morimoto, Akihiro
	Sakashita, Gyosuke
	Urano, Takeshi
	Uchiyama, Susumu
	Fukui, Kiichi
DC.publisher	The Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-01-17
DC.identifier	https://doi.org/10.1242/jcs.063347
DC.subject	Aurora-A
	Kinetochore-microtubule attachment
	Mitosis
	Nucleolin
	SAF-A
	Spindle assembly
	TPX2
DC.description	Ma N., Matsunaga S., Morimoto A., et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. Journal of Cell Science, 124, 3, 394. https://doi.org/10.1242/jcs.063347.
DCTERMS.abstract	Segregation of chromosomes during cell division requires correct formation of mitotic spindles. Here, we show that a scaffold attachment factor A (SAF-A), also known as heterogeneous nuclear ribonucleoprotein-U, contributes to the attachment of spindle microtubules (MTs) to kinetochores and spindle organization. During mitosis, SAF-A was localized at the spindles, spindle midzone and cytoplasmic bridge. Depletion of SAF-A by RNA interference induced mitotic delay and defects in chromosome alignment and spindle assembly. We found that SAF-A specifically co-immunoprecipitated with the chromosome peripheral protein nucleolin and the spindle regulators Aurora-A and TPX2, indicating that SAF-A is associated with nucleolin and the Aurora-A-TPX2 complex. SAF-A was colocalized with TPX2 and Aurora-A in spindle poles and MTs. Elimination of TPX2 or Aurora-A from cells abolished the association of SAF-A with the mitotic spindle. Interestingly, SAF-A can bind to MTs and contributes to the targeting of Aurora-A to mitotic spindle MTs. Our finding indicates that SAF-A is a novel spindle regulator that plays an essential role in kinetochore-MT attachment and mitotic spindle organization.
DC.format	79039
citation_title	The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles
citation_author	Ma, Nan
	Matsunaga, Sachihiro
	Morimoto, Akihiro
	Sakashita, Gyosuke
	Urano, Takeshi
	Uchiyama, Susumu
	Fukui, Kiichi
citation_publisher	The Company of Biologists Ltd
citation_language	English
citation_date	2011-01-17
citation_journal_title	Journal of Cell Science
citation_volume	124
citation_issue	3
citation_firstpage	394
citation_lastpage	404
citation_doi	https://doi.org/10.1242/jcs.063347
citation_pmid	21242313
citation_public_url	https://hdl.handle.net/11094/79039

The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles

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