Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

Ozeki, Yasuyuki; Kitagawa, Yuma; Sumimura, Kazuhiko; Nishizawa, Norihiko; Umemura, Wataru; Kajiyama, Shin'ichiro; Fukui, Kiichi; Itoh, Kazuyoshi

doi:https://doi.org/10.1364/OE.18.013708

Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

Ozeki, Yasuyuki; Kitagawa, Yuma; Sumimura, Kazuhiko et al.

Optics Express, 2010, 18(13), 13708-13719

Number of Access:646（2025-08-15 01:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79041

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Title	Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses
Creator	NRID 1000060437374 1000060437374 CreatorName Ozeki, Yasuyuki
	CreatorName Kitagawa, Yuma
	NRID 1000000452475 1000000452475 CreatorName Sumimura, Kazuhiko
	NRID 1000030273288 1000030273288 CreatorName Nishizawa, Norihiko
	CreatorName Umemura, Wataru
	NRID 1000020243496 1000020243496 CreatorName Kajiyama, Shin'ichiro
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
	NRID 1000080113520 1000080113520 CreatorName Itoh, Kazuyoshi
Description	Ozeki Y., Kitagawa Y., Sumimura K., et al. Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses. Optics Express, 18, 13, 13708. https://doi.org/10.1364/OE.18.013708.
Abstract	We propose and demonstrate the use of subharmonically synchronized laser pulses for low-noise lock-in detection in stimulated Raman scattering (SRS) microscopy. In the experiment, Yb-fiber laser pulses at a repetition rate of 38 MHz are successfully synchronized to Ti:sapphire laser pulses at a repetition rate of 76 MHz with a jitter of <8 fs by a two-photon detector and an intra-cavity electro-optic modulator. By using these pulses, high-frequency lock-in detection of SRS signal is accomplished without high-speed optical modulation. The noise level of the lock-in signal is found to be higher than the shot noise limit only by 1.6 dB. We also demonstrate high-contrast, 3D imaging of unlabeled living cells.
Publisher	Optical Society of American
Source Title	Optics Express
Volume (Issue)	18(13)
Page	13708-13719
Date of Issued	2010-06-21
Language	English
Handle URL	https://hdl.handle.net/11094/79041
eISSN	10944087
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1364/OE.18.013708
Relation.isIdenticalTo	PMID 20588505
Access Rights	open access
CopyRight	©2010 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Optics Express. 2010, 18(13), p. 13708-13719
DC.title	Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses
DC.creator	Ozeki, Yasuyuki
	Kitagawa, Yuma
	Sumimura, Kazuhiko
	Nishizawa, Norihiko
	Umemura, Wataru
	Kajiyama, Shin'ichiro
	Fukui, Kiichi
	Itoh, Kazuyoshi
DC.publisher	Optical Society of American
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-06-21
DC.identifier	https://doi.org/10.1364/OE.18.013708
DC.description	Ozeki Y., Kitagawa Y., Sumimura K., et al. Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses. Optics Express, 18, 13, 13708. https://doi.org/10.1364/OE.18.013708.
DCTERMS.abstract	We propose and demonstrate the use of subharmonically synchronized laser pulses for low-noise lock-in detection in stimulated Raman scattering (SRS) microscopy. In the experiment, Yb-fiber laser pulses at a repetition rate of 38 MHz are successfully synchronized to Ti:sapphire laser pulses at a repetition rate of 76 MHz with a jitter of <8 fs by a two-photon detector and an intra-cavity electro-optic modulator. By using these pulses, high-frequency lock-in detection of SRS signal is accomplished without high-speed optical modulation. The noise level of the lock-in signal is found to be higher than the shot noise limit only by 1.6 dB. We also demonstrate high-contrast, 3D imaging of unlabeled living cells.
DC.rights	©2010 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
DC.format	79041
citation_title	Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses
citation_author	Ozeki, Yasuyuki
	Kitagawa, Yuma
	Sumimura, Kazuhiko
	Nishizawa, Norihiko
	Umemura, Wataru
	Kajiyama, Shin'ichiro
	Fukui, Kiichi
	Itoh, Kazuyoshi
citation_publisher	Optical Society of American
citation_language	English
citation_date	2010-06-21
citation_journal_title	Optics Express
citation_volume	18
citation_issue	13
citation_firstpage	13708
citation_lastpage	13719
citation_doi	https://doi.org/10.1364/OE.18.013708
citation_pmid	20588505
citation_public_url	https://hdl.handle.net/11094/79041

Stimulated raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

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