The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA

Hayashihara, Kayoko; Uchiyama, Susumu; Shimamoto, Shigeru; Kobayashi, Shouhei; Tomschik, Miroslav; Wakamatsu, Hidekazu; No, Daisuke; Sugahara, Hiroki; Hori, Naoto; Noda, Masanori; Ohkubo, Tadayasu; Zlatanova, Jordanka; Matsunaga, Sachihiro; Fukui, Kiichi

doi:https://doi.org/10.1074/jbc.M109.092833

The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA

Hayashihara, Kayoko; Uchiyama, Susumu; Shimamoto, Shigeru et al.

Journal of Biological Chemistry, 2010, 285(9), 6498-6507

Number of Access:1,284（2025-08-15 01:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79042

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Title	The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA
Creator	NRID 1000000624389 1000000624389 CreatorName Hayashihara, Kayoko
	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000000610487 1000000610487 CreatorName Shimamoto, Shigeru
	NRID 1000040425765 1000040425765 CreatorName Kobayashi, Shouhei
	CreatorName Tomschik, Miroslav
	CreatorName Wakamatsu, Hidekazu
	CreatorName No, Daisuke
	CreatorName Sugahara, Hiroki
	CreatorName Hori, Naoto
	CreatorName Noda, Masanori
	NRID 1000090272997 1000090272997 researchmap permalink read0191274 read0191274 Scopus Author ID 7201618682 7201618682 CreatorName Ohkubo, Tadayasu
	CreatorName Zlatanova, Jordanka
	NRID 1000040323448 1000040323448 CreatorName Matsunaga, Sachihiro
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Description	Kayoko Hayashihara, Susumu Uchiyama, Shigeru Shimamoto, Shouhei Kobayashi, Miroslav Tomschik, Hidekazu Wakamatsu, Daisuke No, Hiroki Sugahara, Naoto Hori, Masanori Noda, Tadayasu Ohkubo, Jordanka Zlatanova, Sachihiro Matsunaga, Kiichi Fukui. The Middle Region of an HP1-binding Protein, HP1-BP74, Associates with Linker DNA at the Entry/Exit Site of Nucleosomal DNA. Journal of Biological Chemistry, Volume 285, Issue 9, 2010, Pages 6498-6507. https://doi.org/10.1074/jbc.M109.092833.
Abstract	In higher eukaryotic cells, DNA molecules are present as chromatin fibers, complexes of DNA with various types of proteins; chromatin fibers are highly condensed in metaphase chromosomes during mitosis. Although the formation of the metaphase chromosome structure is essential for the equal segregation of replicated chromosomal DNA into the daughter cells, the mechanism involved in the organization of metaphase chromosomes is poorly understood. To identify proteins involved in the formation and/or maintenance of metaphase chromosomes, we examined proteins that dissociated from isolated human metaphase chromosomes by 0.4 M NaCl treatment; this treatment led to significant chromosome decondensation, but the structure retained the core histones. One of the proteins identified, HP1-BP74 (heterochromatin protein 1-binding protein 74), composed of 553 amino acid residues, was further characterized. HP1-BP74 middle region (BP74Md), composed of 178 amino acid residues (Lys⁹⁷-Lys²⁷⁴), formed a chromatosome-like structure with reconstituted mononucleosomes and protected the linker DNA from micrococcal nuclease digestion by ∼25 bp. The solution structure determined byNMRrevealed that the globular domain (Met¹⁵³-Thr²³⁷) located within BP74Md possesses a structure similar to that of the globular domain of linker histones, which underlies its nucleosome binding properties. Moreover, we confirmed that BP74Md and full-length HP1-BP74 directly binds to HP1 (heterochromatin protein 1) and identified the exact sites responsible for this interaction. Thus, we discovered that HP1-BP74 directly binds to HP1, and its middle region associates with linker DNA at the entry/exit site of nucleosomal DNA in vitro.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	285(9)
Page	6498-6507
Date of Issued	2010-02-26
Language	English
Handle URL	https://hdl.handle.net/11094/79042
PISSN	00219258
eISSN	1083351X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M109.092833
Relation.isIdenticalTo	PMID 20042602
Access Rights	open access
CopyRight	© 2010 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2010, 285(9), p. 6498-6507
DC.title	The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA
DC.creator	Hayashihara, Kayoko
	Uchiyama, Susumu
	Shimamoto, Shigeru
	Kobayashi, Shouhei
	Tomschik, Miroslav
	Wakamatsu, Hidekazu
	No, Daisuke
	Sugahara, Hiroki
	Hori, Naoto
	Noda, Masanori
	Ohkubo, Tadayasu
	Zlatanova, Jordanka
	Matsunaga, Sachihiro
	Fukui, Kiichi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-02-26
DC.identifier	https://doi.org/10.1074/jbc.M109.092833
DC.description	Kayoko Hayashihara, Susumu Uchiyama, Shigeru Shimamoto, Shouhei Kobayashi, Miroslav Tomschik, Hidekazu Wakamatsu, Daisuke No, Hiroki Sugahara, Naoto Hori, Masanori Noda, Tadayasu Ohkubo, Jordanka Zlatanova, Sachihiro Matsunaga, Kiichi Fukui. The Middle Region of an HP1-binding Protein, HP1-BP74, Associates with Linker DNA at the Entry/Exit Site of Nucleosomal DNA. Journal of Biological Chemistry, Volume 285, Issue 9, 2010, Pages 6498-6507. https://doi.org/10.1074/jbc.M109.092833.
DCTERMS.abstract	In higher eukaryotic cells, DNA molecules are present as chromatin fibers, complexes of DNA with various types of proteins; chromatin fibers are highly condensed in metaphase chromosomes during mitosis. Although the formation of the metaphase chromosome structure is essential for the equal segregation of replicated chromosomal DNA into the daughter cells, the mechanism involved in the organization of metaphase chromosomes is poorly understood. To identify proteins involved in the formation and/or maintenance of metaphase chromosomes, we examined proteins that dissociated from isolated human metaphase chromosomes by 0.4 M NaCl treatment; this treatment led to significant chromosome decondensation, but the structure retained the core histones. One of the proteins identified, HP1-BP74 (heterochromatin protein 1-binding protein 74), composed of 553 amino acid residues, was further characterized. HP1-BP74 middle region (BP74Md), composed of 178 amino acid residues (Lys⁹⁷-Lys²⁷⁴), formed a chromatosome-like structure with reconstituted mononucleosomes and protected the linker DNA from micrococcal nuclease digestion by ∼25 bp. The solution structure determined byNMRrevealed that the globular domain (Met¹⁵³-Thr²³⁷) located within BP74Md possesses a structure similar to that of the globular domain of linker histones, which underlies its nucleosome binding properties. Moreover, we confirmed that BP74Md and full-length HP1-BP74 directly binds to HP1 (heterochromatin protein 1) and identified the exact sites responsible for this interaction. Thus, we discovered that HP1-BP74 directly binds to HP1, and its middle region associates with linker DNA at the entry/exit site of nucleosomal DNA in vitro.
DC.rights	© 2010 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	79042
citation_title	The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA
citation_author	Hayashihara, Kayoko
	Uchiyama, Susumu
	Shimamoto, Shigeru
	Kobayashi, Shouhei
	Tomschik, Miroslav
	Wakamatsu, Hidekazu
	No, Daisuke
	Sugahara, Hiroki
	Hori, Naoto
	Noda, Masanori
	Ohkubo, Tadayasu
	Zlatanova, Jordanka
	Matsunaga, Sachihiro
	Fukui, Kiichi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2010-02-26
citation_journal_title	Journal of Biological Chemistry
citation_volume	285
citation_issue	9
citation_firstpage	6498
citation_lastpage	6507
citation_doi	https://doi.org/10.1074/jbc.M109.092833
citation_pmid	20042602
citation_public_url	https://hdl.handle.net/11094/79042

The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA

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