Nucleolin functions in nucleolus formation and chromosome congression

Ma, Nan; Matsunaga, Sachihiro; Takata, Hideaki; Ono, Rika; Uchiyama, Susumu; Fukui, Kiichi

doi:https://doi.org/10.1242/jcs.008771

Nucleolin functions in nucleolus formation and chromosome congression

Ma, Nan; Matsunaga, Sachihiro; Takata, Hideaki et al.

Journal of Cell Science, 2007, 120(12), 2091-2105

Number of Access:708（2025-08-14 03:30 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79046

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Title	Nucleolin functions in nucleolus formation and chromosome congression
Creator	CreatorName Ma, Nan
	NRID 1000040323448 1000040323448 CreatorName Matsunaga, Sachihiro
	NRID 1000020455207 1000020455207 CreatorName Takata, Hideaki
	CreatorName Ono, Rika Creator.Alternative Maniwa, Rika
	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Subject	Nucleolin
	Nucleolus formation
	Chromosome congression
	Mitosis
Description	Ma N., Matsunaga S., Takata H., et al. Nucleolin functions in nucleolus formation and chromosome congression. Journal of Cell Science, 120, 12, 2091. https://doi.org/10.1242/jcs.008771.
Abstract	A complex structure, designated the chromosome periphery, surrounds each chromosome during mitosis. Although several proteins have been shown to localize to the chromosome periphery, their functions during mitosis remain unclear. Here, we used a combination of high-resolution microscopy and RNA-interference-mediated depletion to study the functions of nucleolin, a nucleolar protein localized at the chromosome periphery, in interphase and mitosis. During mitosis, nucleolin was localized in the peripheral region including the vicinity of the outer kinetochore of chromosomes. Staining with an antibody specific for nucleolin phosphorylated by CDC2 revealed that nucleolin was also associated with the spindle poles from prometaphase to anaphase. Nucleolin depletion resulted in disorganization of the nucleoli at interphase. Furthermore, nucleolin-depleted cells showed a prolonged cell cycle with misaligned chromosomes and defects in spindle organization. The misaligned chromosomes showed syntelic kinetochore-microtubule attachments with reduced centromere stretching. Taken together, our results indicate that nucleolin is required for nucleolus formation, and is also involved in chromosome congression and spindle formation.
Publisher	The Company of Biologists Ltd
Source Title	Journal of Cell Science
Volume (Issue)	120(12)
Page	2091-2105
Date of Issued	2007-06-05
Language	English
Handle URL	https://hdl.handle.net/11094/79046
PISSN	00219533
NCID	AA00694823
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1242/jcs.008771
Relation.isIdenticalTo	PMID 17535846
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Cell Science. 2007, 120(12), p. 2091-2105
DC.title	Nucleolin functions in nucleolus formation and chromosome congression
DC.creator	Ma, Nan
	Matsunaga, Sachihiro
	Takata, Hideaki
	Ono, Rika
	Uchiyama, Susumu
	Fukui, Kiichi
DC.creator	Maniwa, Rika
DC.publisher	The Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2007-06-05
DC.identifier	https://doi.org/10.1242/jcs.008771
DC.subject	Nucleolin
	Nucleolus formation
	Chromosome congression
	Mitosis
DC.description	Ma N., Matsunaga S., Takata H., et al. Nucleolin functions in nucleolus formation and chromosome congression. Journal of Cell Science, 120, 12, 2091. https://doi.org/10.1242/jcs.008771.
DCTERMS.abstract	A complex structure, designated the chromosome periphery, surrounds each chromosome during mitosis. Although several proteins have been shown to localize to the chromosome periphery, their functions during mitosis remain unclear. Here, we used a combination of high-resolution microscopy and RNA-interference-mediated depletion to study the functions of nucleolin, a nucleolar protein localized at the chromosome periphery, in interphase and mitosis. During mitosis, nucleolin was localized in the peripheral region including the vicinity of the outer kinetochore of chromosomes. Staining with an antibody specific for nucleolin phosphorylated by CDC2 revealed that nucleolin was also associated with the spindle poles from prometaphase to anaphase. Nucleolin depletion resulted in disorganization of the nucleoli at interphase. Furthermore, nucleolin-depleted cells showed a prolonged cell cycle with misaligned chromosomes and defects in spindle organization. The misaligned chromosomes showed syntelic kinetochore-microtubule attachments with reduced centromere stretching. Taken together, our results indicate that nucleolin is required for nucleolus formation, and is also involved in chromosome congression and spindle formation.
DC.format	79046
citation_title	Nucleolin functions in nucleolus formation and chromosome congression
citation_author	Ma, Nan
	Matsunaga, Sachihiro
	Takata, Hideaki
	Ono, Rika
	Uchiyama, Susumu
	Fukui, Kiichi
citation_publisher	The Company of Biologists Ltd
citation_language	English
citation_date	2007-06-05
citation_journal_title	Journal of Cell Science
citation_volume	120
citation_issue	12
citation_firstpage	2091
citation_lastpage	2105
citation_doi	https://doi.org/10.1242/jcs.008771
citation_pmid	17535846
citation_public_url	https://hdl.handle.net/11094/79046

Nucleolin functions in nucleolus formation and chromosome congression

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