Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator

Shimada, Tomoko; Watanabe, Wataru; Matsunaga, Sachihiro et al.

Optics Express, 2005, 13(24), 9869-9880

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Identifier to cite or link to this item: https://hdl.handle.net/11094/79048

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Title
Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator
Creator
CreatorName
Shimada, Tomoko
NRID
1000090314377 1000090314377
CreatorName
Watanabe, Wataru
NRID
1000040323448 1000040323448
CreatorName
Matsunaga, Sachihiro
NRID
1000090453018 1000090453018
CreatorName
Higashi, Tsunehito
CreatorName
Ishii, Hiroshi
NRID
1000000311770 1000000311770
researchmap permalink
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CreatorName
Fukui, Kiichi
CreatorName
Isobe, Keisuke
NRID
1000080113520 1000080113520
CreatorName
Itoh, Kazuyoshi
Description
Shimada T., Watanabe W., Matsunaga S., et al. Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator. Optics Express, 13, 24, 9869. https://doi.org/10.1364/OPEX.13.009869.
Abstract
Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The longterm viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability.
Publisher
Optical Society of American
Source Title
Optics Express
Volume (Issue)
13(24)
Page
9869-9880
Date of Issued
2005-11-28
Language
English
Handle URL
https://hdl.handle.net/11094/79048
eISSN
10944087
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1364/OPEX.13.009869
Access Rights
open access
CopyRight
©2005 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article