Proteome analysis of human metaphase chromosomes

Uchiyama, Susumu; Kobayashi, Shouhei; Takata, Hideaki; Ishihara, Takeshi; Hori, Naoto; Higashi, Tsunehito; Hayashihara, Kayoko; Sone, Takefumi; Higo, Daisuke; Nirasawa, Takashi; Takao, Toshifumi; Matsunaga, Sachihiro; Fukui, Kiichi

doi:https://doi.org/10.1074/jbc.M412774200

Proteome analysis of human metaphase chromosomes

Uchiyama, Susumu; Kobayashi, Shouhei; Takata, Hideaki et al.

Journal of Biological Chemistry, 2005, 280(17), 16994-17004

Number of Access:1,534（2025-08-14 04:28 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/79049

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Title	Proteome analysis of human metaphase chromosomes
Creator	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 CreatorName Uchiyama, Susumu
	NRID 1000040425765 1000040425765 CreatorName Kobayashi, Shouhei
	NRID 1000020455207 1000020455207 CreatorName Takata, Hideaki
	CreatorName Ishihara, Takeshi
	CreatorName Hori, Naoto
	NRID 1000090453018 1000090453018 CreatorName Higashi, Tsunehito
	NRID 1000000624389 1000000624389 CreatorName Hayashihara, Kayoko
	CreatorName Sone, Takefumi
	CreatorName Higo, Daisuke
	CreatorName Nirasawa, Takashi
	NRID 1000010197048 1000010197048 researchmap permalink Toshifumi_Takao Toshifumi_Takao Scopus Author ID 7102574661 7102574661 CreatorName Takao, Toshifumi
	NRID 1000040323448 1000040323448 CreatorName Matsunaga, Sachihiro
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 CreatorName Fukui, Kiichi
Description	Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Takeshi Ishihara, Naoto Hori, Tsunehito Higashi, Kayoko Hayashihara, Takefumi Sone, Daisuke Higo, Takashi Nirasawa, Toshifumi Takao, Sachihiro Matsunaga, Kiichi Fukui. Proteome Analysis of Human Metaphase Chromosomes. Journal of Biological Chemistry, Volume 280, Issue 17, 2005, Pages 16994-17004. https://doi.org/10.1074/jbc.M412774200.
Abstract	DNA is packaged as chromatin in the interphase nucleus. Dining mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite > 1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into sis groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein frame-work for future research on this topic.
Publisher	American Society for Biochemistry and Molecular Biology
Source Title	Journal of Biological Chemistry
Volume (Issue)	280(17)
Page	16994-17004
Date of Issued	2005-04-29
Language	English
Handle URL	https://hdl.handle.net/11094/79049
PISSN	00219258
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1074/jbc.M412774200
Relation.isIdenticalTo	PMID 15687487
Access Rights	open access
CopyRight	© 2005 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2005, 280(17), p. 16994-17004
DC.title	Proteome analysis of human metaphase chromosomes
DC.creator	Uchiyama, Susumu
	Kobayashi, Shouhei
	Takata, Hideaki
	Ishihara, Takeshi
	Hori, Naoto
	Higashi, Tsunehito
	Hayashihara, Kayoko
	Sone, Takefumi
	Higo, Daisuke
	Nirasawa, Takashi
	Takao, Toshifumi
	Matsunaga, Sachihiro
	Fukui, Kiichi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2005-04-29
DC.identifier	https://doi.org/10.1074/jbc.M412774200
DC.description	Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Takeshi Ishihara, Naoto Hori, Tsunehito Higashi, Kayoko Hayashihara, Takefumi Sone, Daisuke Higo, Takashi Nirasawa, Toshifumi Takao, Sachihiro Matsunaga, Kiichi Fukui. Proteome Analysis of Human Metaphase Chromosomes. Journal of Biological Chemistry, Volume 280, Issue 17, 2005, Pages 16994-17004. https://doi.org/10.1074/jbc.M412774200.
DCTERMS.abstract	DNA is packaged as chromatin in the interphase nucleus. Dining mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite > 1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into sis groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein frame-work for future research on this topic.
DC.rights	© 2005 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	79049
citation_title	Proteome analysis of human metaphase chromosomes
citation_author	Uchiyama, Susumu
	Kobayashi, Shouhei
	Takata, Hideaki
	Ishihara, Takeshi
	Hori, Naoto
	Higashi, Tsunehito
	Hayashihara, Kayoko
	Sone, Takefumi
	Higo, Daisuke
	Nirasawa, Takashi
	Takao, Toshifumi
	Matsunaga, Sachihiro
	Fukui, Kiichi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	English
citation_date	2005-04-29
citation_journal_title	Journal of Biological Chemistry
citation_volume	280
citation_issue	17
citation_firstpage	16994
citation_lastpage	17004
citation_doi	https://doi.org/10.1074/jbc.M412774200
citation_pmid	15687487
citation_public_url	https://hdl.handle.net/11094/79049

Proteome analysis of human metaphase chromosomes

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