Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance

Ogi, Hirotsugu; Fukunishi, Yuji; Omori, Toshinobu; Hatanaka, Kenichi; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1021/ac800459g

Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance

Ogi, Hirotsugu; Fukunishi, Yuji; Omori, Toshinobu et al.

Analytical Chemistry, 2008, 80(14), 5494-5500

Number of Access:639（2025-10-09 11:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84138

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Title	Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 CreatorName Ogi, Hirotsugu
	CreatorName Fukunishi, Yuji
	CreatorName Omori, Toshinobu
	CreatorName Hatanaka, Kenichi
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
	CreatorName Nishiyama, Masayoshi
Description	Hirotsugu Ogi, Yuji Fukunishi, Toshinobu Omori, Kenichi Hatanaka, Masahiko Hirao, and Masayoshi Nishiyama. Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance. Analytical Chemistry, 2008, 80(14), 5494-5500. ©2008 American Chemical Society. https://doi.org/10.1021/ac800459g.
Abstract	In this paper, we developed a 55-MHz wireless-electrodeless quartz crystal microbalance (QCM) and systematically studied the effects of flow rate on the sensitivity to the detection of proteins and on the affinity between biomolecules evaluated by the flow injection system. Brownian motion of proteins in liquid suggests a low probability of meeting, and the convection effect plays an important role in the sensitivity and the affinity in the flow cell injection system. The wireless quartz crystal was isolated in the QCM cell, and flow rates between 50 and 1000 μL/min were used for monitoring binding reactions between human immunoglobulin G and Staphylococcus aureus protein A. The sensitivity was significantly increased as the flow rate increased, while the affinity value remained unchanged. However, the affinity value was affected by the reaction time for a large-concentration analyte, indicating the need of a high-sensitivity biosensor system for accurate evaluation of affinity. The electrode effect on the QCM sensitivity was also theoretically investigated, showing that the electrode significantly deteriorates the QCM sensitivity and makes the Sauerbrey equation invalid.
Publisher	American Chemical Society
Source Title	Analytical Chemistry
Volume (Issue)	80(14)
Page	5494-5500
Date of Issued	2008-07-01
Language	English
Handle URL	https://hdl.handle.net/11094/84138
PISSN	00032700
eISSN	15206882
NCID	AA00524878
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/ac800459g
Relation.isIdenticalTo	PMID 18553940
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac800459g.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analytical Chemistry. 2008, 80(14), p. 5494-5500
DC.title	Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance
DC.creator	Ogi, Hirotsugu
	Fukunishi, Yuji
	Omori, Toshinobu
	Hatanaka, Kenichi
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2008-07-01
DC.identifier	https://doi.org/10.1021/ac800459g
DC.description	Hirotsugu Ogi, Yuji Fukunishi, Toshinobu Omori, Kenichi Hatanaka, Masahiko Hirao, and Masayoshi Nishiyama. Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance. Analytical Chemistry, 2008, 80(14), 5494-5500. ©2008 American Chemical Society. https://doi.org/10.1021/ac800459g.
DCTERMS.abstract	In this paper, we developed a 55-MHz wireless-electrodeless quartz crystal microbalance (QCM) and systematically studied the effects of flow rate on the sensitivity to the detection of proteins and on the affinity between biomolecules evaluated by the flow injection system. Brownian motion of proteins in liquid suggests a low probability of meeting, and the convection effect plays an important role in the sensitivity and the affinity in the flow cell injection system. The wireless quartz crystal was isolated in the QCM cell, and flow rates between 50 and 1000 μL/min were used for monitoring binding reactions between human immunoglobulin G and Staphylococcus aureus protein A. The sensitivity was significantly increased as the flow rate increased, while the affinity value remained unchanged. However, the affinity value was affected by the reaction time for a large-concentration analyte, indicating the need of a high-sensitivity biosensor system for accurate evaluation of affinity. The electrode effect on the QCM sensitivity was also theoretically investigated, showing that the electrode significantly deteriorates the QCM sensitivity and makes the Sauerbrey equation invalid.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac800459g.
DC.format	84138
citation_title	Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance
citation_author	Ogi, Hirotsugu
	Fukunishi, Yuji
	Omori, Toshinobu
	Hatanaka, Kenichi
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	American Chemical Society
citation_language	English
citation_date	2008-07-01
citation_journal_title	Analytical Chemistry
citation_volume	80
citation_issue	14
citation_firstpage	5494
citation_lastpage	5500
citation_doi	https://doi.org/10.1021/ac800459g
citation_pmid	18553940
citation_public_url	https://hdl.handle.net/11094/84138

Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance

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