170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1021/ac901267b

170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji et al.

Analytical Chemistry, 2009, 81(19), 8068-8073

Number of Access:563（2025-10-09 11:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84140

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Title	170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 CreatorName Ogi, Hirotsugu
	CreatorName Nagai, Hironao
	CreatorName Fukunishi, Yuji
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
	CreatorName Nishiyama, Masayoshi
Description	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Masahiko Hirao, and Masayoshi Nishiyama. 170-MHz electrodeless quartz crystal microbalance biosensor: Capability and limitation of higher frequency measurement. Analytical Chemistry, 2009, 81(19), 8068-8073. ©2009 American Chemical Society. https://doi.org/10.1021/ac901267b.
Abstract	We develop a highly sensitive quartz crystal microbalance (QCM) biosensor with a fundamental resonance frequency of 170 MHz. A naked AT-cut quartz plate of 9.7 μm thick is set in a sensor cell. Its shear vibration is excited by the line wire, and the vibration signals are detected by the other line wire, achieving the noncontacting measurement of the resonance frequency. The mass sensitivity of the 170 MHz QCM biosensor is 15 pg/(cm² Hz), which is better than that of a conventional 5 MHz QCM by 3 orders of magnitude. Its high sensitivity is confirmed by detecting human immunoglobulin G (hIgG) via Staphylococcus protein A immobilized nonspecifically on both surfaces of the quartz plate. The detection limit is 0.5 pM. Limitation of the high-frequency QCM measurement is then theoretically discussed with a continuum mechanics model for a plate with point masses connected by elastic springs. The result indicates that a QCM measurement will break down at frequencies one-order-of-magnitude higher than the local resonance frequency at specific binding cites.
Publisher	American Chemical Society
Source Title	Analytical Chemistry
Volume (Issue)	81(19)
Page	8068-8073
Date of Issued	2009-10-01
Language	English
Handle URL	https://hdl.handle.net/11094/84140
PISSN	00032700
eISSN	15206882
NCID	AA00524878
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/ac901267b
Relation.isIdenticalTo	PMID 19728731
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac901267b.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analytical Chemistry. 2009, 81(19), p. 8068-8073
DC.title	170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement
DC.creator	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2009-10-01
DC.identifier	https://doi.org/10.1021/ac901267b
DC.description	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Masahiko Hirao, and Masayoshi Nishiyama. 170-MHz electrodeless quartz crystal microbalance biosensor: Capability and limitation of higher frequency measurement. Analytical Chemistry, 2009, 81(19), 8068-8073. ©2009 American Chemical Society. https://doi.org/10.1021/ac901267b.
DCTERMS.abstract	We develop a highly sensitive quartz crystal microbalance (QCM) biosensor with a fundamental resonance frequency of 170 MHz. A naked AT-cut quartz plate of 9.7 μm thick is set in a sensor cell. Its shear vibration is excited by the line wire, and the vibration signals are detected by the other line wire, achieving the noncontacting measurement of the resonance frequency. The mass sensitivity of the 170 MHz QCM biosensor is 15 pg/(cm² Hz), which is better than that of a conventional 5 MHz QCM by 3 orders of magnitude. Its high sensitivity is confirmed by detecting human immunoglobulin G (hIgG) via Staphylococcus protein A immobilized nonspecifically on both surfaces of the quartz plate. The detection limit is 0.5 pM. Limitation of the high-frequency QCM measurement is then theoretically discussed with a continuum mechanics model for a plate with point masses connected by elastic springs. The result indicates that a QCM measurement will break down at frequencies one-order-of-magnitude higher than the local resonance frequency at specific binding cites.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac901267b.
DC.format	84140
citation_title	170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement
citation_author	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	American Chemical Society
citation_language	English
citation_date	2009-10-01
citation_journal_title	Analytical Chemistry
citation_volume	81
citation_issue	19
citation_firstpage	8068
citation_lastpage	8073
citation_doi	https://doi.org/10.1021/ac901267b
citation_pmid	19728731
citation_public_url	https://hdl.handle.net/11094/84140

170-MHz electrodeless quartz crystal microbalance biosensor : Capability and limitation of higher frequency measurement

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