Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji; Yanagida, Taiji; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1143/JJAP.49.07HD07

Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji et al.

Japanese Journal of Applied Physics, 2010, 49, 07HD07

Number of Access:369（2025-11-25 02:19 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84165

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Title	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 ORCID 0000-0002-9602-5194 0000-0002-9602-5194 CreatorName Ogi, Hirotsugu
	CreatorName Nagai, Hironao
	CreatorName Fukunishi, Yuji
	CreatorName Yanagida, Taiji
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
	CreatorName Nishiyama, Masayoshi
Description	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Taiji Yanagida, Masahiko Hirao and Masayoshi Nishiyama. Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins. Japanese Journal of Applied Physics, 2010, 49(7S), 07HD07. https://doi.org/10.1143/JJAP.49.07HD07.
Abstract	Staphylococcus aureus protein A (SPA) shows high nonspecific binding affinity on a naked quartz surface, and it can be used as the receptor protein for detecting immunoglobulin G (IgG), the most important immunoglobulin. The immunosensor ability, however, significantly depends on the immobilization procedure. In this work, the effect of the nonspecific immobilization procedure on the sensor sensitivity is studied using a homebuilt electrodeless quartz-crystal microbalance (QCM) biosensor. The pure-shear vibration of a 9.7-mm-thick AT-cut quartz plate is excited and detected in liquids by the line antenna located outside the flow channel. SPA molecules are immobilized on the quartz surfaces, and human IgG is injected to monitor the binding reaction between SPA and IgG. This study reveals that a long (nearly 24 h) immersion procedure is required for immobilizing SPA to achieve the tight biding with the quartz surfaces.
Publisher	The Japan Society of Applied Physics
Source Title	Japanese Journal of Applied Physics
Volume (Issue)	49
Page	07HD07
Date of Issued	2010-07-20
Language	English
Handle URL	https://hdl.handle.net/11094/84165
PISSN	00214922
eISSN	13474065
NCID	AA12295836
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1143/JJAP.49.07HD07
Access Rights	open access
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Japanese Journal of Applied Physics. 2010, 49, p. 07HD07
DC.title	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
DC.creator	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Yanagida, Taiji
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	The Japan Society of Applied Physics
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-07-20
DC.identifier	https://doi.org/10.1143/JJAP.49.07HD07
DC.description	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Taiji Yanagida, Masahiko Hirao and Masayoshi Nishiyama. Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins. Japanese Journal of Applied Physics, 2010, 49(7S), 07HD07. https://doi.org/10.1143/JJAP.49.07HD07.
DCTERMS.abstract	Staphylococcus aureus protein A (SPA) shows high nonspecific binding affinity on a naked quartz surface, and it can be used as the receptor protein for detecting immunoglobulin G (IgG), the most important immunoglobulin. The immunosensor ability, however, significantly depends on the immobilization procedure. In this work, the effect of the nonspecific immobilization procedure on the sensor sensitivity is studied using a homebuilt electrodeless quartz-crystal microbalance (QCM) biosensor. The pure-shear vibration of a 9.7-mm-thick AT-cut quartz plate is excited and detected in liquids by the line antenna located outside the flow channel. SPA molecules are immobilized on the quartz surfaces, and human IgG is injected to monitor the binding reaction between SPA and IgG. This study reveals that a long (nearly 24 h) immersion procedure is required for immobilizing SPA to achieve the tight biding with the quartz surfaces.
DC.format	84165
citation_title	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
citation_author	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Yanagida, Taiji
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	The Japan Society of Applied Physics
citation_language	English
citation_date	2010-07-20
citation_journal_title	Japanese Journal of Applied Physics
citation_volume	49
citation_firstpage	07HD07
citation_doi	https://doi.org/10.1143/JJAP.49.07HD07
citation_public_url	https://hdl.handle.net/11094/84165

Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

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