Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito et al.

Japanese Journal of Applied Physics, 2015, 54(9), 096601

Number of Access:396（2025-10-09 11:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84172

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
161332_1 pdf None 1.13 MB 227 2021.09.14  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation
Creator
CreatorName
Shagawa, Tomohiro
CreatorName
Torii, Hiroomi
NRID
1000070780170 1000070780170
CreatorName
Kato, Fumihito
NRID
1000090252626 1000090252626
researchmap permalink
read0042771 read0042771
Scopus Author ID
35563683500 35563683500
CreatorName
Ogi, Hirotsugu
NRID
1000080112027 1000080112027
CreatorName
Hirao, Masahiko
Description
Tomohiro Shagawa, Hiroomi Torii, Fumihito Kato, Hirotsugu Ogi and Masahiko Hirao. Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation. Japanese Journal of Applied Physics, 2015, 54(9), 096601. https://doi.org/10.7567/JJAP.54.096601.
Abstract
In this study, we demonstrate the effectiveness of a resonance acoustic microbalance with a naked embedded quartz (RAMNE-Q) biosensor for evaluating viscoelastic property changes in thin protein layers during protein deposition reactions without dissipation measurement. Quartz crystal microbalance (QCM) biosensors have conventionally been adopted for the viscoelasticity evaluation of adsorbed protein layers by measuring dissipation as well as resonance frequency. However, dissipation, or the vibrational energy loss, is easily affected by many factors and is rarely measured with sufficiently high accuracy. To evaluate viscoelasticity only from a reliable frequency response, one needs to perform an ultrahighfrequency measurement, which is here achieved using the RAMNE-Q biosensor. Simultaneous frequency measurement is performed for fundamental and overtone modes up to 406MHz of a 58MHz RAMNE-Q biosensor during various binding reactions, and evolutions of viscosity, shear modulus, and thickness of adsorbed protein layers are inversely evaluated. A marked difference is observed in the viscosity evolution between specific and nonspecific binding reactions. Furthermore, the reversed frequency response appears, which indicates the modification of the protein structure into a rigid structure.
Publisher
The Japan Society of Applied Physics
Source Title
Japanese Journal of Applied Physics
Volume (Issue)
54(9)
Page
096601
Date of Issued
2015-08-06
Language
English
Handle URL
https://hdl.handle.net/11094/84172
PISSN
00214922
eISSN
13474065
NCID
AA12295836
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.7567/JJAP.54.096601
Access Rights
open access
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article