Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukunishi, Yuji; Yanagida, Taiji; Yagi, Hisashi; Goto, Yuji; Fukushima, Masahiko; Uesugi, Kentaro; Hirao, Masahiko

doi:https://doi.org/10.1021/ac2007703

Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukunishi, Yuji; Yanagida, Taiji et al.

Analytical Chemistry, 2011, 83(12), 4982-4988

Number of Access:509（2025-10-09 11:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84185

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Title	Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 CreatorName Ogi, Hirotsugu
	CreatorName Fukunishi, Yuji
	CreatorName Yanagida, Taiji
	NRID 1000010432494 1000010432494 CreatorName Yagi, Hisashi
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
	CreatorName Fukushima, Masahiko
	CreatorName Uesugi, Kentaro
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
Description	Ogi H., Fukunishi Y., Yanagida T., et al. "Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor", Analytical Chemistry, 83(12), 4982-4988, May 11, 2011. Copyright © 2011 American Chemical Society. https://doi.org/10.1021/ac2007703.
Abstract	Real-time monitoring of the deposition processes of Aβ1-40 and Aβ1-42 peptides on various seeds has been performed using a 55 MHz wireless quartz-crystal microbalance (QCM) over long-time periods (∼40 h). Dissolved peptide solutions were stirred for nucleation and growth of seeds at pH = 7.4 and 4.6, which were immobilized on the sensor chips. The isolated Aβ peptides were then flowed at the neutral pH, focusing on the interaction between the seeds and the monomers (or small multimers), excluding other interactions among seeds and other aggregates. The thioflavin-T fluorescence assay and atomic-force microscopy were used for evaluating structures of the seeds and deposited aggregates. The deposition rate, determined by the frequency decrease, is about 100 monomers/nm2/year in the case of fibril formation. The notable deposition behavior was observed in the deposition of Aβ1-40 peptide on Aβ1-42 seeds grown at the lower pH, which can be an important model for Alzheimer's disease. © 2011 American Chemical Society.
Publisher	American Chemical Society
Source Title	Analytical Chemistry
Volume (Issue)	83(12)
Page	4982-4988
Date of Issued	2011-05-11
Language	English
Handle URL	https://hdl.handle.net/11094/84185
PISSN	00032700
NCID	AA00524878
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1021/ac2007703
Relation.isIdenticalTo	PMID 21557621
Access Rights	open access
CopyRight	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac2007703.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analytical Chemistry. 2011, 83(12), p. 4982-4988
DC.title	Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor
DC.creator	Ogi, Hirotsugu
	Fukunishi, Yuji
	Yanagida, Taiji
	Yagi, Hisashi
	Goto, Yuji
	Fukushima, Masahiko
	Uesugi, Kentaro
	Hirao, Masahiko
DC.publisher	American Chemical Society
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-05-11
DC.identifier	https://doi.org/10.1021/ac2007703
DC.description	Ogi H., Fukunishi Y., Yanagida T., et al. "Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor", Analytical Chemistry, 83(12), 4982-4988, May 11, 2011. Copyright © 2011 American Chemical Society. https://doi.org/10.1021/ac2007703.
DCTERMS.abstract	Real-time monitoring of the deposition processes of Aβ1-40 and Aβ1-42 peptides on various seeds has been performed using a 55 MHz wireless quartz-crystal microbalance (QCM) over long-time periods (∼40 h). Dissolved peptide solutions were stirred for nucleation and growth of seeds at pH = 7.4 and 4.6, which were immobilized on the sensor chips. The isolated Aβ peptides were then flowed at the neutral pH, focusing on the interaction between the seeds and the monomers (or small multimers), excluding other interactions among seeds and other aggregates. The thioflavin-T fluorescence assay and atomic-force microscopy were used for evaluating structures of the seeds and deposited aggregates. The deposition rate, determined by the frequency decrease, is about 100 monomers/nm2/year in the case of fibril formation. The notable deposition behavior was observed in the deposition of Aβ1-40 peptide on Aβ1-42 seeds grown at the lower pH, which can be an important model for Alzheimer's disease. © 2011 American Chemical Society.
DC.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac2007703.
DC.format	84185
citation_title	Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor
citation_author	Ogi, Hirotsugu
	Fukunishi, Yuji
	Yanagida, Taiji
	Yagi, Hisashi
	Goto, Yuji
	Fukushima, Masahiko
	Uesugi, Kentaro
	Hirao, Masahiko
citation_publisher	American Chemical Society
citation_language	English
citation_date	2011-05-11
citation_journal_title	Analytical Chemistry
citation_volume	83
citation_issue	12
citation_firstpage	4982
citation_lastpage	4988
citation_doi	https://doi.org/10.1021/ac2007703
citation_pmid	21557621
citation_public_url	https://hdl.handle.net/11094/84185

Seed-dependent deposition behavior of Aβ peptides studied with wireless quartz-crystal-microbalance biosensor

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