Replacement-free mass-amplified sandwich assay with 180-MHz electrodeless quartz-crystal microbalance biosensor

Ogi, Hirotsugu; Yanagida, Taiji; Hirao, Masahiko et al.

Biosensors and Bioelectronics, 2011, 26(12), 4819-4822

Number of Access:271（2025-10-09 11:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84194

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Title
Replacement-free mass-amplified sandwich assay with 180-MHz electrodeless quartz-crystal microbalance biosensor
Creator
NRID
1000090252626 1000090252626
researchmap permalink
read0042771 read0042771
Scopus Author ID
35563683500 35563683500
CreatorName
Ogi, Hirotsugu
CreatorName
Yanagida, Taiji
NRID
1000080112027 1000080112027
CreatorName
Hirao, Masahiko
CreatorName
Nishiyama, Masayoshi
Subject
CRP
Electrodeless QCM
Replacement free
Sensitivity amplification
Abstract
This study describes a sensitivity-amplified detection method in a replacement-free electrodeless quartz-crystal microbalance (QCM) biosensor. A sandwich assay is proposed for detecting C-reactive proteins (CRP), where the biotinated second anti-CRP antibody is weighted by streptavidin for sensitivity amplification. Because the first CRP antibody was immobilized nonspecifically on naked quartz surfaces, the sandwich assay was repeated using the same sensor chip, making possible the replacement-free assay. The mass-amplified sandwich assay detected a CRP solution of 0.1. ng/ml. A methodology for determining the molecular mass of the injected protein is also proposed. © 2011 Elsevier B.V.
Publisher
Elsevier B.V.
Source Title
Biosensors and Bioelectronics
Volume (Issue)
26(12)
Page
4819-4822
Date of Issued
2011-05-30
Language
English
Handle URL
https://hdl.handle.net/11094/84194
PISSN
09565663
eISSN
18734235
NCID
AA10739666
AA1152282X
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1016/j.bios.2011.05.035
Relation.isIdenticalTo
PMID
21680171
Access Rights
open access
CopyRight
© 2011 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article