Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukushima, Masahiko; Uesugi, Kentaro; Yagi, Hisashi; Goto, Yuji; Hirao, Masahiko

doi:https://doi.org/10.1016/j.bios.2012.07.019

Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukushima, Masahiko; Uesugi, Kentaro et al.

Biosensors and Bioelectronics, 2012, 40(1), 200-205

Number of Access:418（2025-11-25 02:19 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84196

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Title	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 ORCID 0000-0002-9602-5194 0000-0002-9602-5194 CreatorName Ogi, Hirotsugu
	CreatorName Fukushima, Masahiko
	CreatorName Uesugi, Kentaro
	NRID 1000010432494 1000010432494 CreatorName Yagi, Hisashi
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
Subject	Aβ
	Deposition rate
	Oligomer
	QCM
	Ultrasonication
Abstract	High-frequency (~ 55MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of AΒ1-40 peptide on AΒ1-42 nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction was monitored over 40h, and the deposition rate was deduced. Among the agitation nuclei, the maximum deposition rate was observed on the nucleus grown at pH 4.6. However, ultrasonication nucleus grown at pH 7.4 produced much larger deposition rate, despite the same Β-sheet concentration. This result indicates that local structural modulation is caused in the nucleus by ultrasonication, which adsorbs the AΒ peptide more actively than other nuclei. The resultant deposits clearly show oligomeric structure. © 2012 Elsevier B.V.
Publisher	Elsevier B.V.
Source Title	Biosensors and Bioelectronics
Volume (Issue)	40(1)
Page	200-205
Date of Issued	2012-07-21
Language	English
Handle URL	https://hdl.handle.net/11094/84196
PISSN	09565663
eISSN	18734235
NCID	AA10739666
NCID	AA1152282X
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1016/j.bios.2012.07.019
Relation.isIdenticalTo	PMID 22857904
Access Rights	open access
CopyRight	© 2012 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biosensors and Bioelectronics. 2012, 40(1), p. 200-205
DC.title	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
DC.creator	Ogi, Hirotsugu
	Fukushima, Masahiko
	Uesugi, Kentaro
	Yagi, Hisashi
	Goto, Yuji
	Hirao, Masahiko
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2012-07-21
DC.identifier	https://doi.org/10.1016/j.bios.2012.07.019
DC.subject	Aβ
	Deposition rate
	Oligomer
	QCM
	Ultrasonication
DCTERMS.abstract	High-frequency (~ 55MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of AΒ1-40 peptide on AΒ1-42 nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction was monitored over 40h, and the deposition rate was deduced. Among the agitation nuclei, the maximum deposition rate was observed on the nucleus grown at pH 4.6. However, ultrasonication nucleus grown at pH 7.4 produced much larger deposition rate, despite the same Β-sheet concentration. This result indicates that local structural modulation is caused in the nucleus by ultrasonication, which adsorbs the AΒ peptide more actively than other nuclei. The resultant deposits clearly show oligomeric structure. © 2012 Elsevier B.V.
DC.rights	© 2012 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	84196
citation_title	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
citation_author	Ogi, Hirotsugu
	Fukushima, Masahiko
	Uesugi, Kentaro
	Yagi, Hisashi
	Goto, Yuji
	Hirao, Masahiko
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2012-07-21
citation_journal_title	Biosensors and Bioelectronics
citation_volume	40
citation_issue	1
citation_firstpage	200
citation_lastpage	205
citation_doi	https://doi.org/10.1016/j.bios.2012.07.019
citation_pmid	22857904
citation_public_url	https://hdl.handle.net/11094/84196

Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

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