Drastic acceleration of fibrillation of insulin by transient cavitation bubble

Nakajima, Kichitaro; Nishioka, Daisuke; Hirao, Masahiko; So, Masatomo; Goto, Yuji; Ogi, Hirotsugu

doi:https://doi.org/10.1016/j.ultsonch.2016.11.034

Drastic acceleration of fibrillation of insulin by transient cavitation bubble

Nakajima, Kichitaro; Nishioka, Daisuke; Hirao, Masahiko et al.

Ultrasonics Sonochemistry, 2016, 36, 206-211

Number of Access:604（2025-10-09 08:17 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84200

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Title	Drastic acceleration of fibrillation of insulin by transient cavitation bubble
Creator	NRID 1000020867337 1000020867337 ORCID 0000-0003-2644-741X 0000-0003-2644-741X researchmap permalink sonochem sonochem Scopus Author ID 57145904700 57145904700 CreatorName Nakajima, Kichitaro
	CreatorName Nishioka, Daisuke
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
	NRID 1000040746335 1000040746335 CreatorName So, Masatomo
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 CreatorName Goto, Yuji
	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 CreatorName Ogi, Hirotsugu
Subject	Acoustic field analysis
	Aggregation acceleration
	Amyloid fibril
	Subharmonic wave
	Transient bubbles
Description	Nakajima K., Nishioka D., Hirao M., et al. Drastic acceleration of fibrillation of insulin by transient cavitation bubble. Ultrasonics Sonochemistry, 36, 206, 2016. https://doi.org/10.1016/j.ultsonch.2016.11.034.
Abstract	Amyloid-fibril formation of proteins can be accelerated by ultrasonic irradiation to the peptide solutions. Although this phenomenon contributes to understanding pathogenic behavior of amyloidosis, its physical mechanism has not been clarified, because several factors (cavitation, temperature increase, stirring effect, and so on) related to ultrasonic irradiation can participate in the fibrillation reaction. Here, we independently study contributions of the possible factors, using insulin, which is extremely stable and then suitable for the mechanism clarification. We find that the optimized ultrasonic irradiation can drastically accelerate the fibrillation reaction; the time for completing the reaction is shortened compared with the high-speed (1200 rpm) stirring agitation by a factor of 430. The fibrillation reaction proceeds only when the subharmonic-mode intensity exceeds a threshold, indicating generation of the transient cavitation bubbles. Our results reveal that not the temperature increase but the transient cavitation bubbles work as the dominant accelerator of the fibrillation reaction.
Publisher	Elsevier B.V.
Source Title	Ultrasonics Sonochemistry
Volume (Issue)	36
Page	206-211
Date of Issued	2016-11-28
Language	English
Handle URL	https://hdl.handle.net/11094/84200
PISSN	13504177
eISSN	18732828
NCID	AA11024447
NCID	AA11541041
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.ultsonch.2016.11.034
Relation.isIdenticalTo	PMID 28069203
Access Rights	open access
CopyRight	© 2016 Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Ultrasonics Sonochemistry. 2016, 36, p. 206-211
DC.title	Drastic acceleration of fibrillation of insulin by transient cavitation bubble
DC.creator	Nakajima, Kichitaro
	Nishioka, Daisuke
	Hirao, Masahiko
	So, Masatomo
	Goto, Yuji
	Ogi, Hirotsugu
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2016-11-28
DC.identifier	https://doi.org/10.1016/j.ultsonch.2016.11.034
DC.subject	Acoustic field analysis
	Aggregation acceleration
	Amyloid fibril
	Subharmonic wave
	Transient bubbles
DC.description	Nakajima K., Nishioka D., Hirao M., et al. Drastic acceleration of fibrillation of insulin by transient cavitation bubble. Ultrasonics Sonochemistry, 36, 206, 2016. https://doi.org/10.1016/j.ultsonch.2016.11.034.
DCTERMS.abstract	Amyloid-fibril formation of proteins can be accelerated by ultrasonic irradiation to the peptide solutions. Although this phenomenon contributes to understanding pathogenic behavior of amyloidosis, its physical mechanism has not been clarified, because several factors (cavitation, temperature increase, stirring effect, and so on) related to ultrasonic irradiation can participate in the fibrillation reaction. Here, we independently study contributions of the possible factors, using insulin, which is extremely stable and then suitable for the mechanism clarification. We find that the optimized ultrasonic irradiation can drastically accelerate the fibrillation reaction; the time for completing the reaction is shortened compared with the high-speed (1200 rpm) stirring agitation by a factor of 430. The fibrillation reaction proceeds only when the subharmonic-mode intensity exceeds a threshold, indicating generation of the transient cavitation bubbles. Our results reveal that not the temperature increase but the transient cavitation bubbles work as the dominant accelerator of the fibrillation reaction.
DC.rights	© 2016 Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DC.format	84200
citation_title	Drastic acceleration of fibrillation of insulin by transient cavitation bubble
citation_author	Nakajima, Kichitaro
	Nishioka, Daisuke
	Hirao, Masahiko
	So, Masatomo
	Goto, Yuji
	Ogi, Hirotsugu
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2016-11-28
citation_journal_title	Ultrasonics Sonochemistry
citation_volume	36
citation_firstpage	206
citation_lastpage	211
citation_doi	https://doi.org/10.1016/j.ultsonch.2016.11.034
citation_pmid	28069203
citation_public_url	https://hdl.handle.net/11094/84200

Drastic acceleration of fibrillation of insulin by transient cavitation bubble

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