Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

Ogi, Hirotsugu; Motohisa, Kazuma; Hatanaka, Kenichi; Ohmori, Toshinobu; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1016/j.bios.2007.03.003

Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

Ogi, Hirotsugu; Motohisa, Kazuma; Hatanaka, Kenichi et al.

Biosensors and Bioelectronics, 2007, 22(12), 3238-3242

Number of Access:1,019（2025-11-25 02:19 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/84472

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
Bios_22_12_3238	pdf	None	357 KB	322	2021.09.17

Creative Commons : Attribution - NonCommercial - NoDerivatives

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
Creator	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 ORCID 0000-0002-9602-5194 0000-0002-9602-5194 CreatorName Ogi, Hirotsugu
	CreatorName Motohisa, Kazuma
	CreatorName Hatanaka, Kenichi
	CreatorName Ohmori, Toshinobu
	NRID 1000080112027 1000080112027 CreatorName Hirao, Masahiko
	CreatorName Nishiyama, Masayoshi
Subject	Affinity
	Electrodeless
	QCM
	IgG
	Protein A
	Wireless
Abstract	The binding affinity between human immunoglobulin G (IgG) and protein A was studied by the homebuilt wireless-electrodeless quartz crystal microbalance (QCM). Protein A was immobilized on the electrodeless AT-cut quartz plate of 0.05 mm thick and its fundamental resonance frequency near 34 MHz was measured by a noncontacting manner using a line antenna. The vibrational analysis was performed to ensure higher sensitivity of the electrodeless QCM. A flow-cell system was fabricated to continuously measure the resonance frequency during the injection sequence of the IgG solutions with concentrations of 1-20,000 ng/mL. The exponential frequency changes were recorded to determine the affinity based on the Langmuir kinetics. The equilibrium constant KA significantly varied between 6×10⁶ and 6×10¹⁰ M-¹, depending on the IgG concentration, which is attributed to various formations of IgG-protein A complexes.
Publisher	Elsevier B.V.
Source Title	Biosensors and Bioelectronics
Volume (Issue)	22(12)
Page	3238-3242
Date of Issued	2007-06-15
Language	English
Handle URL	https://hdl.handle.net/11094/84472
PISSN	09565663
NCID	AA10739666
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1016/j.bios.2007.03.003
Relation.isIdenticalTo	PMID 17420120
Access Rights	open access
CopyRight	© 2007 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biosensors and Bioelectronics. 2007, 22(12), p. 3238-3242
DC.title	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
DC.creator	Ogi, Hirotsugu
	Motohisa, Kazuma
	Hatanaka, Kenichi
	Ohmori, Toshinobu
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2007-06-15
DC.identifier	https://doi.org/10.1016/j.bios.2007.03.003
DC.subject	Affinity
	Electrodeless
	QCM
	IgG
	Protein A
	Wireless
DCTERMS.abstract	The binding affinity between human immunoglobulin G (IgG) and protein A was studied by the homebuilt wireless-electrodeless quartz crystal microbalance (QCM). Protein A was immobilized on the electrodeless AT-cut quartz plate of 0.05 mm thick and its fundamental resonance frequency near 34 MHz was measured by a noncontacting manner using a line antenna. The vibrational analysis was performed to ensure higher sensitivity of the electrodeless QCM. A flow-cell system was fabricated to continuously measure the resonance frequency during the injection sequence of the IgG solutions with concentrations of 1-20,000 ng/mL. The exponential frequency changes were recorded to determine the affinity based on the Langmuir kinetics. The equilibrium constant KA significantly varied between 6×10⁶ and 6×10¹⁰ M-¹, depending on the IgG concentration, which is attributed to various formations of IgG-protein A complexes.
DC.rights	© 2007 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	84472
citation_title	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
citation_author	Ogi, Hirotsugu
	Motohisa, Kazuma
	Hatanaka, Kenichi
	Ohmori, Toshinobu
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2007-06-15
citation_journal_title	Biosensors and Bioelectronics
citation_volume	22
citation_issue	12
citation_firstpage	3238
citation_lastpage	3242
citation_doi	https://doi.org/10.1016/j.bios.2007.03.003
citation_pmid	17420120
citation_public_url	https://hdl.handle.net/11094/84472

Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

Link to primary information

Item Information

Show details