Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

Koike, Kota; Smith, Nicholas; Fujita, Katsumasa

doi:https://doi.org/10.1117/12.2609736

Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

Koike, Kota; Smith, Nicholas; Fujita, Katsumasa

Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2022, 11973, 1197308

Number of Access:554（2025-12-01 03:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/88581

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Title	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
Creator	CreatorName Koike, Kota
	NRID 1000010362592 1000010362592 ORCID 0000-0002-7835-199X 0000-0002-7835-199X researchmap permalink read0089935 read0089935 Scopus Author ID 55262815400 55262815400 CreatorName Smith, Nicholas
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 CreatorName Fujita, Katsumasa
Subject	label-free
	pulse shaping
	Raman scattering
	spectral focusing
	stimulated Raman scattering microscopy
Description	SPIE BIOS, 22 January - 28 February 2022, San Francisco, California, United States
Abstract	We propose the use of 14 ps chirped laser pulses in stimulated Raman scattering (SRS) microscopy to improve the spectral resolution and signal-to-background ratio (SBR) in SRS imaging. We developed a single-grating-based pulse chirper and implemented it into an intensity-modulation SRS microscope to stretch the excitation pulse width from 2 to 14 ps. We confirmed that the 14 ps pulses provide a spectral resolution of 2 cm-1 by measuring the SRS spectra of diamond crystals. We found that the 14 ps pulses have smaller nonlinear background signals and improve SBR in SRS imaging of various samples due to the instantaneous narrow-band excitation and low peak power. Our technique can broaden the application of the 2 ps intensity modulation SRS microscopy by improving the spectral resolution and sensitivity.
Publisher	SPIE
Source Title	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume (Issue)	11973
Page	1197308
Date of Issued	2022-03-02
Language	English
Handle URL	https://hdl.handle.net/11094/88581
PISSN	16057422
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1117/12.2609736
Relation.isIdenticalTo	NCID BA51116822
Access Rights	open access
oaire:version	Version of Record
Category	会議発表論文 Conference Paper

Resource Type	conference paper
Local Type	Conference Paper
DCMI Type	Text
DCTERMS.bibliographicCitation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2022, 11973, p. 1197308
DC.title	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
DC.creator	Koike, Kota
	Smith, Nicholas
	Fujita, Katsumasa
DC.publisher	SPIE
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-03-02
DC.identifier	https://doi.org/10.1117/12.2609736
DC.subject	label-free
	pulse shaping
	Raman scattering
	spectral focusing
	stimulated Raman scattering microscopy
DC.description	SPIE BIOS, 22 January - 28 February 2022, San Francisco, California, United States
DCTERMS.abstract	We propose the use of 14 ps chirped laser pulses in stimulated Raman scattering (SRS) microscopy to improve the spectral resolution and signal-to-background ratio (SBR) in SRS imaging. We developed a single-grating-based pulse chirper and implemented it into an intensity-modulation SRS microscope to stretch the excitation pulse width from 2 to 14 ps. We confirmed that the 14 ps pulses provide a spectral resolution of 2 cm-1 by measuring the SRS spectra of diamond crystals. We found that the 14 ps pulses have smaller nonlinear background signals and improve SBR in SRS imaging of various samples due to the instantaneous narrow-band excitation and low peak power. Our technique can broaden the application of the 2 ps intensity modulation SRS microscopy by improving the spectral resolution and sensitivity.
DC.format	88581
citation_title	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
citation_author	Koike, Kota
	Smith, Nicholas
	Fujita, Katsumasa
citation_publisher	SPIE
citation_language	English
citation_date	2022-03-02
citation_journal_title	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
citation_volume	11973
citation_firstpage	1197308
citation_doi	https://doi.org/10.1117/12.2609736
citation_public_url	https://hdl.handle.net/11094/88581

Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

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