Evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs

Shogen, Yosuke; Isomura, Emiko; Kogo, Mikihiko

PLoS ONE, 2014, 9(4), e94365

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Identifier to cite or link to this item: https://hdl.handle.net/11094/89688

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Title
Evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs
Creator
NRID
1000080585745 1000080585745
CreatorName
Shogen, Yosuke
NRID
1000070397701 1000070397701
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Scopus Author ID
55667115400 55667115400
ORCID
0000-0002-7855-9600 0000-0002-7855-9600
CreatorName
Isomura, Emiko
Creator.Alternative
Tanaka, Emiko
NRID
1000020205371 1000020205371
CreatorName
Kogo, Mikihiko
Description
Evaluation of Inferior Alveolar Nerve Regeneration by Bifocal Distraction Osteogenesis with Retrograde Transportation of Horseradish Peroxidase in Dogs. Shogen Y, Isomura ET, Kogo M. PLOS ONE 2014. 9(4) e94365. doi:10.1371/journal.pone.0094365
Abstract
Background: Bifocal distraction osteogenesis has been shown to be a reliable method for reconstructing segmental mandibular defects. However, there are few reports regarding the occurrence of inferior alveolar nerve regeneration during the process of distraction. Previously, we reported inferior alveolar nerve regeneration after distraction, and evaluated the regenerated nerve using histological and electrophysiological methods. In the present study, we investigated axons regenerated by bifocal distraction osteogenesis using retrograde transportation of horseradish peroxidase in the mandibles of dogs to determine their type and function. Methods and Findings: Using a bifocal distraction osteogenesis method, we produced a 10-mm mandibular defect, including a nerve defect, in 11 dogs and distracted using a transport disk at a rate of 1 mm/day. The regenerated inferior alveolar nerve was evaluated by retrograde transportation of HRP in all dogs at 3 and 6 months after the first operation. At 3 and 6 months, HRP-labeled neurons were observed in the trigeminal ganglion. The number of HRP-labeled neurons in each section increased, while the cell body diameter of HRP-labeled neurons was reduced over time. Conclusions: We found that the inferior alveolar nerve after bifocal distraction osteogenesis successfully recovered until peripheral tissue began to function. Although our research is still at the stage of animal experiments, it is considered that it will be possible to apply this method in the future to humans who have the mandibular defects. © 2014 Shogen et al.
Publisher
Public Library of Science
Source Title
PLoS ONE
Volume (Issue)
9(4)
Page
e94365
Date of Issued
2014-04-14
Language
English
Handle URL
https://hdl.handle.net/11094/89688
eISSN
19326203
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1371/journal.pone.0094365
Access Rights
open access
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This article is licensed under a Creative Commons Attribution 4.0 International License.
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Category
学術雑誌論文 Journal Article