Ionic Signal Amplification of DNA in a Nanopore

Tsutsui, Makusu; Yokota, Kazumichi; He, Yuhui et al.

Small Methods, 2022, 6(11), 2200761

Number of Access:186（2025-06-06 18:00 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89744

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
SmallMethods_6_2200761 pdf None 2.94 MB 262 2022.12.23  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Ionic Signal Amplification of DNA in a Nanopore
Creator
NRID
1000050546596 1000050546596
ORCID
0000-0002-4552-1163 0000-0002-4552-1163
researchmap permalink
makusu makusu
Scopus Author ID
8978905800 8978905800
CreatorName
Tsutsui, Makusu
NRID
1000050633179 1000050633179
CreatorName
Yokota, Kazumichi
CreatorName
He, Yuhui
NRID
1000020092546 1000020092546
CreatorName
Kawai, Tomoji
Subject
ionic amplification
nanofluidics
nanopores
permittivity gradients
translocation dynamics
Description
This is the pre-peer reviewed version of the following article: Tsutsui, M., Yokota, K., He, Y., Kawai, T., Ionic Signal Amplification of DNA in a Nanopore. Small Methods 2022, 6, 2200761, which has been published in final form at https://doi.org/10.1002/smtd.202200761. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Abstract
Ionic signal amplification is a key challenge for single-molecule analyses by solid-state nanopore sensing. Here, a permittivity gradient approach for amplifying ionic blockade characteristics of DNA in a nanofluidic channel is reported. The transmembrane ionic current response is found to change substantially through modifying the liquid permittivity at one side of a pore with an organic solvent. Imposing positive liquid permittivity gradients with respect to the direction of DNA electrophoresis, this study observes the resistive ionic signals to become larger due to the varying contributions of molecular counterions. On the contrary, negative gradients render adverse effects causing conductive ionic current pulses upon polynucleotide translocations. Most importantly, both the positive and negative gradients are demonstrated to be capable of amplifying the ionic signals by an order of magnitude with a 1.3-fold difference in the transmembrane liquid dielectric constants. This phenomenon allows a novel way to enhance the single-molecule sensitivity of nanopore sensing that may be useful in analyzing secondary structures and genome sequence of DNA by ionic current measurements.
Publisher
Wiley-VCH
Source Title
Small Methods
Volume (Issue)
6(11)
Page
2200761
Date of Issued
2022-11-18
Date of Submitted
2022-06-14
Language
English
Handle URL
https://hdl.handle.net/11094/89744
eISSN
23669608
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1002/smtd.202200761
Access Rights
open access
CopyRight
© 2022 Wiley-VCH GmbH
oaire:version
Author’s Original
Category
学術雑誌論文 Journal Article