A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading

Watanabe, Ryota; Matsugaki, Aira; Ishimoto, Takuya et al.

International Journal of Molecular Sciences, 2022, 23(13), 7423

Number of Access:741（2025-07-18 10:03 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89748

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Title
A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading
Creator
CreatorName
Watanabe, Ryota
NRID
1000010592529 1000010592529
ORCID
0000-0002-8427-7124 0000-0002-8427-7124
researchmap permalink
aira aira
Scopus Author ID
24172720900 24172720900
CreatorName
Matsugaki, Aira
NRID
1000050508835 1000050508835
ORCID
0000-0003-0081-0591 0000-0003-0081-0591
researchmap permalink
i_taku i_taku
Scopus Author ID
35310484700 35310484700
CreatorName
Ishimoto, Takuya
NRID
1000080845347 1000080845347
ORCID
0000-0002-2773-072X 0000-0002-2773-072X
researchmap permalink
ozasa_r ozasa_r
Scopus Author ID
56202812400 56202812400
CreatorName
Ozasa, Ryosuke
NRID
1000040324793 1000040324793
CreatorName
Matsumoto, Takuya
NRID
1000030243182 1000030243182
ORCID
0000-0001-8052-1698 0000-0001-8052-1698
researchmap permalink
read0013987 read0013987
Scopus Author ID
7403896013 7403896013
CreatorName
Nakano, Takayoshi
Subject
collagen/apatite orientation
endochondral ossification
ex vivo
mechanical loading
osteocyte morphology
Description
Watanabe R., Matsugaki A., Ishimoto T., et al. A Novel Ex Vivo Bone Culture Model for Regulation of Collagen/Apatite Preferential Orientation by Mechanical Loading. International Journal of Molecular Sciences, 23, 13, 7423. https://doi.org/10.3390/ijms23137423.
Abstract
The anisotropic microstructure of bone, composed of collagen fibers and biological apatite crystallites, is an important determinant of its mechanical properties. Recent studies have revealed that the preferential orientation of collagen/apatite composites is closely related to the direction and magnitude of in vivo principal stress. However, the mechanism of alteration in the collagen/apatite microstructure to adapt to the mechanical environment remains unclear. In this study, we established a novel ex vivo bone culture system using embryonic mouse femurs, which enabled artificial control of the mechanical environment. The mineralized femur length significantly increased following cultivation; uniaxial mechanical loading promoted chondrocyte hypertrophy in the growth plates of embryonic mouse femurs. Compressive mechanical loading using the ex vivo bone culture system induced a higher anisotropic microstructure than that observed in the unloaded femur. Osteocytes in the anisotropic bone microstructure were elongated and aligned along the long axis of the femur, which corresponded to the principal loading direction. The ex vivo uniaxial mechanical loading successfully induced the formation of an oriented collagen/apatite microstructure via osteocyte mechano-sensation in a manner quite similar to the in vivo environment.
Publisher
MDPI
Source Title
International Journal of Molecular Sciences
Volume (Issue)
23(13)
Page
7423
Date of Issued
2022-07-04
Language
English
Handle URL
https://hdl.handle.net/11094/89748
PISSN
16616596
eISSN
14220067
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.3390/ijms23137423
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article