Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity

Hamai, Ryo; Sakai, Susumu; Shiwaku, Yukari; Anada, Takahisa; Tsuchiya, Kaori; Ishimoto, Takuya; Nakano, Takayoshi; Suzuki, Osamu

doi:https://doi.org/10.1016/j.apmt.2021.101279

Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity

Hamai, Ryo; Sakai, Susumu; Shiwaku, Yukari et al.

Applied Materials Today, 2022, 26, 101279

Number of Access:670（2025-07-18 10:02 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89754

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Title	Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity
Creator	CreatorName Hamai, Ryo
	CreatorName Sakai, Susumu
	CreatorName Shiwaku, Yukari
	CreatorName Anada, Takahisa
	CreatorName Tsuchiya, Kaori
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	NRID 1000060374948 1000060374948 CreatorName Suzuki, Osamu
Subject	Dislocation
	Dissolution
	Lattice strain
	Octacalcium phosphate
	Osteogenecity
Description	Hamai R., Sakai S., Shiwaku Y., et al. Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity. Applied Materials Today, 26, 101279. https://doi.org/10.1016/j.apmt.2021.101279.
Abstract	Herein, we show that the enhanced osteogenecity of octacalcium phosphate (OCP) biomaterial, recently identified as an important element in hybrid organic–inorganic nanocomposites involved in the initial hydroxyapatite crystal expansion in mammal bones, results from an enhanced chemical property, stemming from the presence of lattice strain and dislocations. Two types of OCPs were synthesized by wet-chemical processing in the presence (c-OCP) and absence (w-OCP) of gelatin, respectively, and subjected to structural, chemical, and biological analyses. High-resolution transmission electron microscopy (HRTEM) and fast Fourier transform (FFT) analyses revealed that c-OCP crystals contained approximately six times higher edge dislocations with Burgers vectors perpendicular to a-axis than that in the case of w-OCP. The dissolution of c-OCP crystal in tris-HCl buffer occurred toward the long axis of the crystal, most likely, toward the lattice strain along the c-axis direction, while w-OCP crystal dissolved toward the a-axis direction. The study suggested that the increment of internal energy by the higher dislocation density contributed promoting c-OCP dissolution and hydrolysis through decreasing the activation energy. c-OCP crystal displayed enhanced in vitro mesenchymal stem 2D cell and 3D spheroid differentiation, in vivo bone formation, and apatite crystallographic orientation in critical-sized rat calvarial defect model as compared to w-OCP crystal, at the same time, converting to apatite structure earlier than w-OCP. The present study demonstrates that dislocation-related dissolution along with enhanced conversion of OCP is a determinant in bone induction, which may be relevant to normal bone development utilizing OCP biomaterials.
Publisher	Elsevier
Source Title	Applied Materials Today
Volume (Issue)	26
Page	101279
Date of Issued	2022-03-01
Language	English
Handle URL	https://hdl.handle.net/11094/89754
eISSN	23529407
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.apmt.2021.101279
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Applied Materials Today. 2022, 26, p. 101279
DC.title	Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity
DC.creator	Hamai, Ryo
	Sakai, Susumu
	Shiwaku, Yukari
	Anada, Takahisa
	Tsuchiya, Kaori
	Ishimoto, Takuya
	Nakano, Takayoshi
	Suzuki, Osamu
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-03-01
DC.identifier	https://doi.org/10.1016/j.apmt.2021.101279
DC.subject	Dislocation
	Dissolution
	Lattice strain
	Octacalcium phosphate
	Osteogenecity
DC.description	Hamai R., Sakai S., Shiwaku Y., et al. Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity. Applied Materials Today, 26, 101279. https://doi.org/10.1016/j.apmt.2021.101279.
DCTERMS.abstract	Herein, we show that the enhanced osteogenecity of octacalcium phosphate (OCP) biomaterial, recently identified as an important element in hybrid organic–inorganic nanocomposites involved in the initial hydroxyapatite crystal expansion in mammal bones, results from an enhanced chemical property, stemming from the presence of lattice strain and dislocations. Two types of OCPs were synthesized by wet-chemical processing in the presence (c-OCP) and absence (w-OCP) of gelatin, respectively, and subjected to structural, chemical, and biological analyses. High-resolution transmission electron microscopy (HRTEM) and fast Fourier transform (FFT) analyses revealed that c-OCP crystals contained approximately six times higher edge dislocations with Burgers vectors perpendicular to a-axis than that in the case of w-OCP. The dissolution of c-OCP crystal in tris-HCl buffer occurred toward the long axis of the crystal, most likely, toward the lattice strain along the c-axis direction, while w-OCP crystal dissolved toward the a-axis direction. The study suggested that the increment of internal energy by the higher dislocation density contributed promoting c-OCP dissolution and hydrolysis through decreasing the activation energy. c-OCP crystal displayed enhanced in vitro mesenchymal stem 2D cell and 3D spheroid differentiation, in vivo bone formation, and apatite crystallographic orientation in critical-sized rat calvarial defect model as compared to w-OCP crystal, at the same time, converting to apatite structure earlier than w-OCP. The present study demonstrates that dislocation-related dissolution along with enhanced conversion of OCP is a determinant in bone induction, which may be relevant to normal bone development utilizing OCP biomaterials.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	89754
citation_title	Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity
citation_author	Hamai, Ryo
	Sakai, Susumu
	Shiwaku, Yukari
	Anada, Takahisa
	Tsuchiya, Kaori
	Ishimoto, Takuya
	Nakano, Takayoshi
	Suzuki, Osamu
citation_publisher	Elsevier
citation_language	English
citation_date	2022-03-01
citation_journal_title	Applied Materials Today
citation_volume	26
citation_firstpage	101279
citation_doi	https://doi.org/10.1016/j.apmt.2021.101279
citation_public_url	https://hdl.handle.net/11094/89754

Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity

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