Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

Kawai, Mariko; Ozasa, Ryosuke; Ishimoto, Takuya; Nakano, Takayoshi; Yamamoto, Hiromitsu; Kashiwagi, Marina; Yamanaka, Shigeki; Nakao, Kazumasa; Maruyama, Hiroki; Bessho, Kazuhisa; Ohura, Kiyoshi

doi:https://doi.org/10.3390/ma15030993

Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

Kawai, Mariko; Ozasa, Ryosuke; Ishimoto, Takuya et al.

Materials, 2022, 15(3), 993

Number of Access:1,170（2025-07-18 10:02 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89757

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Title	Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer
Creator	NRID 1000040379839 1000040379839 CreatorName Kawai, Mariko Creator.Alternative Yamamoto, Mariko
	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	CreatorName Yamamoto, Hiromitsu
	CreatorName Kashiwagi, Marina
	CreatorName Yamanaka, Shigeki
	CreatorName Nakao, Kazumasa
	CreatorName Maruyama, Hiroki
	CreatorName Bessho, Kazuhisa
	NRID 1000020131378 1000020131378 CreatorName Ohura, Kiyoshi
Subject	BMP
	Bone quality
	Collagen micro-arrangement
	Gene therapy
	Periodontal tissue
Description	Kawai M.Y., Ozasa R., Ishimoto T., et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15, 3, 993. https://doi.org/10.3390/ma15030993.
Abstract	The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.
Publisher	MDPI
Source Title	Materials
Volume (Issue)	15(3)
Page	993
Date of Issued	2022-01-27
Language	English
Handle URL	https://hdl.handle.net/11094/89757
eISSN	19961944
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.3390/ma15030993
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Materials. 2022, 15(3), p. 993
DC.title	Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer
DC.creator	Kawai, Mariko
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Nakano, Takayoshi
	Yamamoto, Hiromitsu
	Kashiwagi, Marina
	Yamanaka, Shigeki
	Nakao, Kazumasa
	Maruyama, Hiroki
	Bessho, Kazuhisa
	Ohura, Kiyoshi
DC.creator	Yamamoto, Mariko
DC.publisher	MDPI
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-01-27
DC.identifier	https://doi.org/10.3390/ma15030993
DC.subject	BMP
	Bone quality
	Collagen micro-arrangement
	Gene therapy
	Periodontal tissue
DC.description	Kawai M.Y., Ozasa R., Ishimoto T., et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15, 3, 993. https://doi.org/10.3390/ma15030993.
DCTERMS.abstract	The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89757
citation_title	Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer
citation_author	Kawai, Mariko
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Nakano, Takayoshi
	Yamamoto, Hiromitsu
	Kashiwagi, Marina
	Yamanaka, Shigeki
	Nakao, Kazumasa
	Maruyama, Hiroki
	Bessho, Kazuhisa
	Ohura, Kiyoshi
citation_publisher	MDPI
citation_language	English
citation_date	2022-01-27
citation_journal_title	Materials
citation_volume	15
citation_issue	3
citation_firstpage	993
citation_doi	https://doi.org/10.3390/ma15030993
citation_public_url	https://hdl.handle.net/11094/89757

Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

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