Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli

Matsuzaka, Tadaaki; Matsugaki, Aira; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.biomaterials.2021.121203

Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli

Matsuzaka, Tadaaki; Matsugaki, Aira; Nakano, Takayoshi

Biomaterials, 2021, 279, 121203

Number of Access:400（2025-07-27 15:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89764

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Title	Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli
Creator	CreatorName Matsuzaka, Tadaaki
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Anisotropic collagen/apatite microstructure
	Cell orientation
	mechanosensing
	Osteocyte-osteoblast coculture
	PGE2
Description	Matsuzaka T., Matsugaki A., Nakano T.. Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli. Biomaterials, 279, 121203. https://doi.org/10.1016/j.biomaterials.2021.121203.
Abstract	Anisotropic collagen/apatite microstructure is a prominent determinant of bone tissue functionalization; in particular, bone matrix modulates its anisotropic microstructure depending on the surrounding mechanical condition. Although mechanotransduction in bones is governed by osteocyte function, the precise mechanisms linking mechanical stimuli and anisotropic formation of collagen/apatite microstructure are poorly understood. Here we developed a novel anisotropic mechano-coculture system which enables the understanding of the biological mechanisms regulating the oriented bone matrix formation, which is constructed by aligned osteoblasts. The developed model provides bone-mimetic coculture platform that enables simultaneous control of mechanical condition and osteoblast-osteocyte communication with an anisotropic culture scaffold. The engineered coculture device helps in understanding the relationship between osteocyte mechanoresponses and osteoblast arrangement, which is a significant contributor to anisotropic organization of bone tissue. Our study showed that osteocyte responses to oscillatory flow stimuli regulated osteoblast arrangement through soluble molecular interactions. Importantly, we found that prostaglandin E2 is a novel determinant for oriented collagen/apatite organization of bone matrix, through controlling osteoblast arrangement.
Publisher	Elsevier
Source Title	Biomaterials
Volume (Issue)	279
Page	121203
Date of Issued	2021-12-01
Language	English
Handle URL	https://hdl.handle.net/11094/89764
PISSN	01429612
eISSN	18785905
NCID	AA00110092
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.biomaterials.2021.121203
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biomaterials. 2021, 279, p. 121203
DC.title	Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli
DC.creator	Matsuzaka, Tadaaki
	Matsugaki, Aira
	Nakano, Takayoshi
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-12-01
DC.identifier	https://doi.org/10.1016/j.biomaterials.2021.121203
DC.subject	Anisotropic collagen/apatite microstructure
	Cell orientation
	mechanosensing
	Osteocyte-osteoblast coculture
	PGE2
DC.description	Matsuzaka T., Matsugaki A., Nakano T.. Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli. Biomaterials, 279, 121203. https://doi.org/10.1016/j.biomaterials.2021.121203.
DCTERMS.abstract	Anisotropic collagen/apatite microstructure is a prominent determinant of bone tissue functionalization; in particular, bone matrix modulates its anisotropic microstructure depending on the surrounding mechanical condition. Although mechanotransduction in bones is governed by osteocyte function, the precise mechanisms linking mechanical stimuli and anisotropic formation of collagen/apatite microstructure are poorly understood. Here we developed a novel anisotropic mechano-coculture system which enables the understanding of the biological mechanisms regulating the oriented bone matrix formation, which is constructed by aligned osteoblasts. The developed model provides bone-mimetic coculture platform that enables simultaneous control of mechanical condition and osteoblast-osteocyte communication with an anisotropic culture scaffold. The engineered coculture device helps in understanding the relationship between osteocyte mechanoresponses and osteoblast arrangement, which is a significant contributor to anisotropic organization of bone tissue. Our study showed that osteocyte responses to oscillatory flow stimuli regulated osteoblast arrangement through soluble molecular interactions. Importantly, we found that prostaglandin E2 is a novel determinant for oriented collagen/apatite organization of bone matrix, through controlling osteoblast arrangement.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89764
citation_title	Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli
citation_author	Matsuzaka, Tadaaki
	Matsugaki, Aira
	Nakano, Takayoshi
citation_publisher	Elsevier
citation_language	English
citation_date	2021-12-01
citation_journal_title	Biomaterials
citation_volume	279
citation_firstpage	121203
citation_doi	https://doi.org/10.1016/j.biomaterials.2021.121203
citation_public_url	https://hdl.handle.net/11094/89764

Control of osteoblast arrangement by osteocyte mechanoresponse through prostaglandin E2 signaling under oscillatory fluid flow stimuli

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