Quantitative Evaluation of Osteocyte Morphology and Bone Anisotropic Extracellular Matrix in Rat Femur

Ishimoto, Takuya; Kawahara, Keita; Matsugaki, Aira et al.

Calcified Tissue International, 2021, 109, 434-444

Number of Access:462（2025-07-18 09:55 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89769

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Title
Quantitative Evaluation of Osteocyte Morphology and Bone Anisotropic Extracellular Matrix in Rat Femur
Creator
NRID
1000050508835 1000050508835
ORCID
0000-0003-0081-0591 0000-0003-0081-0591
researchmap permalink
i_taku i_taku
Scopus Author ID
35310484700 35310484700
CreatorName
Ishimoto, Takuya
CreatorName
Kawahara, Keita
NRID
1000010592529 1000010592529
ORCID
0000-0002-8427-7124 0000-0002-8427-7124
researchmap permalink
aira aira
Scopus Author ID
24172720900 24172720900
CreatorName
Matsugaki, Aira
NRID
1000080253219 1000080253219
CreatorName
Kamioka, Hiroshi
NRID
1000030243182 1000030243182
ORCID
0000-0001-8052-1698 0000-0001-8052-1698
researchmap permalink
read0013987 read0013987
Scopus Author ID
7403896013 7403896013
CreatorName
Nakano, Takayoshi
Subject
Anisotropy
Apatite orientation
Bone quality
Lacno-canalicular system
Osteocytes
Description
Ishimoto T., Kawahara K., Matsugaki A., et al. Quantitative Evaluation of Osteocyte Morphology and Bone Anisotropic Extracellular Matrix in Rat Femur. Calcified Tissue International, 109, 4, 434. https://doi.org/10.1007/s00223-021-00852-1.
Abstract
Osteocytes are believed to play a crucial role in mechanosensation and mechanotransduction which are important for maintenance of mechanical integrity of bone. Recent investigations have revealed that the preferential orientation of bone extracellular matrix (ECM) mainly composed of collagen fibers and apatite crystallites is one of the important determinants of bone mechanical integrity. However, the relationship between osteocytes and ECM orientation remains unclear. In this study, the association between ECM orientation and anisotropy in the osteocyte lacuno-canalicular system, which is thought to be optimized along with the mechanical stimuli, was investigated using male rat femur. The degree of ECM orientation along the femur longitudinal axis was significantly and positively correlated with the anisotropic features of the osteocyte lacunae and canaliculi. At the femur middiaphysis, there are the osteocytes with lacunae that highly aligned along the bone long axis (principal stress direction) and canaliculi that preferentially extended perpendicular to the bone long axis, and the highest degree of apatite c-axis orientation along the bone long axis was shown. Based on these data, we propose a model in which osteocytes can change their lacuno-canalicular architecture depending on the mechanical environment so that they can become more susceptible to mechanical stimuli via fluid flow in the canalicular channel.
Publisher
Springer
Source Title
Calcified Tissue International
Volume (Issue)
109
Page
434-444
Date of Issued
2021-05-19
Language
English
Handle URL
https://hdl.handle.net/11094/89769
PISSN
0171967X
eISSN
14320827
NCID
AA00141085
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s00223-021-00852-1
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article