Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype

Kuroda, Yukiko; Kawaai, Katsuhiro; Hatano, Naoya; Wu, Yanlin; Takano, Hidekazu; Momose, Atsushi; Ishimoto, Takuya; Nakano, Takayoshi; Roschger, Paul; Blouin, Stéphane; Matsuo, Koichi

doi:https://doi.org/10.1002/jbmr.4320

Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype

Kuroda, Yukiko; Kawaai, Katsuhiro; Hatano, Naoya et al.

Journal of Bone and Mineral Research, 2021, 36(8), 1535-1547

Number of Access:664（2025-07-18 10:08 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89776

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Title	Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype
Creator	NRID 1000070455343 1000070455343 CreatorName Kuroda, Yukiko
	CreatorName Kawaai, Katsuhiro
	CreatorName Hatano, Naoya
	CreatorName Wu, Yanlin
	CreatorName Takano, Hidekazu
	CreatorName Momose, Atsushi
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	CreatorName Roschger, Paul
	CreatorName Blouin, Stéphane
	NRID 1000040229422 1000040229422 CreatorName Matsuo, Koichi
Description	Kuroda Y., Kawaai K., Hatano N., et al. Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype. Journal of Bone and Mineral Research, 36, 8, 1535. https://doi.org/10.1002/jbmr.4320.
Abstract	Auditory ossicles in the middle ear and bony labyrinth of the inner ear are highly mineralized in adult mammals. Cellular mechanisms underlying formation of dense bone during development are unknown. Here, we found that osteoblast-like cells synthesizing highly mineralized hearing-related bones produce both type I and type II collagens as the bone matrix, while conventional osteoblasts and chondrocytes primarily produce type I and type II collagens, respectively. Furthermore, these osteoblast-like cells were not labeled in a “conventional osteoblast”-specific green fluorescent protein (GFP) mouse line. Type II collagen-producing osteoblast-like cells were not chondrocytes as they express osteocalcin, localize along alizarin-labeled osteoid, and form osteocyte lacunae and canaliculi, as do conventional osteoblasts. Auditory ossicles and the bony labyrinth exhibit not only higher bone matrix mineralization but also a higher degree of apatite orientation than do long bones. Therefore, we conclude that these type II collagen-producing hypermineralizing osteoblasts (termed here auditory osteoblasts) represent a new osteoblast subtype. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Publisher	John Wiley and Sons
Source Title	Journal of Bone and Mineral Research
Volume (Issue)	36(8)
Page	1535-1547
Date of Issued	2021-04-27
Language	English
Handle URL	https://hdl.handle.net/11094/89776
PISSN	08840431
eISSN	15234681
NCID	AA10688587
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1002/jbmr.4320
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Bone and Mineral Research. 2021, 36(8), p. 1535-1547
DC.title	Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype
DC.creator	Kuroda, Yukiko
	Kawaai, Katsuhiro
	Hatano, Naoya
	Wu, Yanlin
	Takano, Hidekazu
	Momose, Atsushi
	Ishimoto, Takuya
	Nakano, Takayoshi
	Roschger, Paul
	Blouin, Stéphane
	Matsuo, Koichi
DC.publisher	John Wiley and Sons
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-04-27
DC.identifier	https://doi.org/10.1002/jbmr.4320
DC.description	Kuroda Y., Kawaai K., Hatano N., et al. Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype. Journal of Bone and Mineral Research, 36, 8, 1535. https://doi.org/10.1002/jbmr.4320.
DCTERMS.abstract	Auditory ossicles in the middle ear and bony labyrinth of the inner ear are highly mineralized in adult mammals. Cellular mechanisms underlying formation of dense bone during development are unknown. Here, we found that osteoblast-like cells synthesizing highly mineralized hearing-related bones produce both type I and type II collagens as the bone matrix, while conventional osteoblasts and chondrocytes primarily produce type I and type II collagens, respectively. Furthermore, these osteoblast-like cells were not labeled in a “conventional osteoblast”-specific green fluorescent protein (GFP) mouse line. Type II collagen-producing osteoblast-like cells were not chondrocytes as they express osteocalcin, localize along alizarin-labeled osteoid, and form osteocyte lacunae and canaliculi, as do conventional osteoblasts. Auditory ossicles and the bony labyrinth exhibit not only higher bone matrix mineralization but also a higher degree of apatite orientation than do long bones. Therefore, we conclude that these type II collagen-producing hypermineralizing osteoblasts (termed here auditory osteoblasts) represent a new osteoblast subtype. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89776
citation_title	Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype
citation_author	Kuroda, Yukiko
	Kawaai, Katsuhiro
	Hatano, Naoya
	Wu, Yanlin
	Takano, Hidekazu
	Momose, Atsushi
	Ishimoto, Takuya
	Nakano, Takayoshi
	Roschger, Paul
	Blouin, Stéphane
	Matsuo, Koichi
citation_publisher	John Wiley and Sons
citation_language	English
citation_date	2021-04-27
citation_journal_title	Journal of Bone and Mineral Research
citation_volume	36
citation_issue	8
citation_firstpage	1535
citation_lastpage	1547
citation_doi	https://doi.org/10.1002/jbmr.4320
citation_public_url	https://hdl.handle.net/11094/89776

Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype

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