Effect of precursor deficiency induced ca/p ratio on antibacterial and osteoblast adhesion properties of ag-incorporated hydroxyapatite: Reducing ag toxicity

Gokcekaya, Ozkan; Ergun, Celaletdin; Webster, Thomas J. et al.

Materials, 2021, 14(12), 3158

Number of Access:653（2025-08-21 04:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89782

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Title
Effect of precursor deficiency induced ca/p ratio on antibacterial and osteoblast adhesion properties of ag-incorporated hydroxyapatite: Reducing ag toxicity
Creator
CreatorName
Gokcekaya, Ozkan
CreatorName
Ergun, Celaletdin
CreatorName
Webster, Thomas J.
CreatorName
Bahadir, Abdurrahman
NRID
1000040507901 1000040507901
CreatorName
Ueda, Kyosuke
NRID
1000020198394 1000020198394
CreatorName
Narushima, Takayuki
NRID
1000030243182 1000030243182
ORCID
0000-0001-8052-1698 0000-0001-8052-1698
researchmap permalink
read0013987 read0013987
Scopus Author ID
7403896013 7403896013
CreatorName
Nakano, Takayoshi
Subject
Antibacterial
Carbonate
Hydroxyapatite
Incorporation
Osteoblast adhesion
Silver
Description
Gokcekaya O., Ergun C., Webster T.J., et al. Effect of precursor deficiency induced ca/p ratio on antibacterial and osteoblast adhesion properties of ag-incorporated hydroxyapatite: Reducing ag toxicity. Materials, 14, 12, 3158. https://doi.org/10.3390/ma14123158.
Abstract
Ag-containing hydroxyapatite (HA) can reduce risks associated with bacterial infections which may eventually require additional surgical operations to retrieve a failed implant. The biologi-cal properties of HA in such applications are strongly affected by its composition in terms of dopants as well as Ca/P stoichiometry, which can be easily controlled by altering processing parameters, such as precursor concentrations. The objective of this in vitro study was to understand the effect of variations in HA precursor solutions on antibacterial properties againstEscherichia coli (E. coli) and for promoting osteoblast (bone-forming cell) adhesion on Ag incorporated HA (AgHA) which has not yet been investigated. For this, two groups of AgHAs were synthesized via a precipitation method by adjusting precursor reactants with a stoichiometric value of 1.67, being either (Ca + Ag)/P (Ca-deficient) or Ca/(P + Ag) (P-deficient), and were characterized by XRD, FTIR, and SEM-EDS. Results showed that Ag+ incorporated into the Ca2+ sites was associated with a corresponding OH− vacancy. Additional incorporation of CO32− into PO43− sites occurred specifically for the P-deficient AgHAs. While antibacterial properties increased, osteoblast adhesion decreased with increasing Ag content for the Ca-deficient AgHAs, as anticipated. In contrast, significant antibacterial properties with good osteoblast behavior were observed on the P-deficient AgHAs even with a lower Ag content, owing to carbonated HA. Thus, this showed that by synthesizing AgHA using P-deficient precursors with carbonate substitution, one can keep the antibacterial properties of Ag in HA while reducing its toxic effect on osteoblasts.
Publisher
MDPI
Source Title
Materials
Volume (Issue)
14(12)
Page
3158
Date of Issued
2021-06-08
Language
English
Handle URL
https://hdl.handle.net/11094/89782
eISSN
19961944
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.3390/ma14123158
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article