Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold

Ozasa, Ryosuke; Matsugaki, Aira; Matsuzaka, Tadaaki; Ishimoto, Takuya; Yun, Hui Suk; Nakano, Takayoshi

doi:https://doi.org/10.3390/ijms22126232

Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold

Ozasa, Ryosuke; Matsugaki, Aira; Matsuzaka, Tadaaki et al.

International Journal of Molecular Sciences, 2021, 22(12), 6232

Number of Access:554（2025-07-18 10:06 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89783

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
IntJMolSci_22_12	pdf	None	3.27 MB	86	2022.12.26

Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold
Creator	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	CreatorName Matsuzaka, Tadaaki
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	CreatorName Yun, Hui Suk
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Bone regenerative medicine
	Cell proliferation
	Cell therapy
	Cellular arrangement
	Focal adhesion
	Induced pluripotent stem cell
	Osteoblast
Description	Ozasa R., Matsugaki A., Matsuzaka T., et al. Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold. International Journal of Molecular Sciences, 22, 12, 6232. https://doi.org/10.3390/ijms22126232.
Abstract	Human-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. Recently, we revealed that hiPSC-derived osteoblasts (hiPSC-Obs) exhibit preferential alignment and organize in highly ordered bone matrices along a bone-mimetic collagen scaffold, indicating their critical role in regulating the unidirectional cellular arrangement, as well as the structural organization of regenerated bone tissue. However, it remains unclear how hiPSCs exhibit the cell properties required for oriented tissue construction. The present study aimed to characterize the properties of hiPSCs-Obs and those of their focal adhesions (FAs), which mediate the structural relationship between cells and the matrix. Our in vitro anisotropic cell culture system revealed the superior adhesion behavior of hiPSC-Obs, which exhibited accelerated cell proliferation and better cell alignment along the collagen axis compared to normal human osteoblasts. Notably, the oriented collagen scaffold stimulated FA formation along the scaffold collagen orientation. This is the first report of the superior cell adhesion behavior of hiPSC-Obs associated with the promotion of FA assembly along an anisotropic scaffold. These findings suggest a promising role for hiPSCs in enabling anisotropic bone microstructural regeneration.
Publisher	MDPI
Source Title	International Journal of Molecular Sciences
Volume (Issue)	22(12)
Page	6232
Date of Issued	2021-06-09
Language	English
Handle URL	https://hdl.handle.net/11094/89783
PISSN	16616596
eISSN	14220067
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.3390/ijms22126232
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	International Journal of Molecular Sciences. 2021, 22(12), p. 6232
DC.title	Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold
DC.creator	Ozasa, Ryosuke
	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Ishimoto, Takuya
	Yun, Hui Suk
	Nakano, Takayoshi
DC.publisher	MDPI
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-06-09
DC.identifier	https://doi.org/10.3390/ijms22126232
DC.subject	Bone regenerative medicine
	Cell proliferation
	Cell therapy
	Cellular arrangement
	Focal adhesion
	Induced pluripotent stem cell
	Osteoblast
DC.description	Ozasa R., Matsugaki A., Matsuzaka T., et al. Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold. International Journal of Molecular Sciences, 22, 12, 6232. https://doi.org/10.3390/ijms22126232.
DCTERMS.abstract	Human-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. Recently, we revealed that hiPSC-derived osteoblasts (hiPSC-Obs) exhibit preferential alignment and organize in highly ordered bone matrices along a bone-mimetic collagen scaffold, indicating their critical role in regulating the unidirectional cellular arrangement, as well as the structural organization of regenerated bone tissue. However, it remains unclear how hiPSCs exhibit the cell properties required for oriented tissue construction. The present study aimed to characterize the properties of hiPSCs-Obs and those of their focal adhesions (FAs), which mediate the structural relationship between cells and the matrix. Our in vitro anisotropic cell culture system revealed the superior adhesion behavior of hiPSC-Obs, which exhibited accelerated cell proliferation and better cell alignment along the collagen axis compared to normal human osteoblasts. Notably, the oriented collagen scaffold stimulated FA formation along the scaffold collagen orientation. This is the first report of the superior cell adhesion behavior of hiPSC-Obs associated with the promotion of FA assembly along an anisotropic scaffold. These findings suggest a promising role for hiPSCs in enabling anisotropic bone microstructural regeneration.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89783
citation_title	Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold
citation_author	Ozasa, Ryosuke
	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Ishimoto, Takuya
	Yun, Hui Suk
	Nakano, Takayoshi
citation_publisher	MDPI
citation_language	English
citation_date	2021-06-09
citation_journal_title	International Journal of Molecular Sciences
citation_volume	22
citation_issue	12
citation_firstpage	6232
citation_doi	https://doi.org/10.3390/ijms22126232
citation_public_url	https://hdl.handle.net/11094/89783

Superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold

Link to primary information

Item Information

Show details