Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells

Okada, Rintaro; Yamato, Kai; Kawakami, Minoru; Kodama, Joe; Kushioka, Junichi; Tateiwa, Daisuke; Ukon, Yuichiro; Zeynep, Bal; Ishimoto, Takuya; Nakano, Takayoshi; Yoshikawa, Hideki; Kaito, Takashi

doi:https://doi.org/10.1016/j.bonr.2021.100757

Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells

Okada, Rintaro; Yamato, Kai; Kawakami, Minoru et al.

Bone Reports, 2021, 14, 100757

Number of Access:1,223（2025-07-18 10:03 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89784

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Title	Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells
Creator	CreatorName Okada, Rintaro
	CreatorName Yamato, Kai
	CreatorName Kawakami, Minoru
	CreatorName Kodama, Joe
	CreatorName Kushioka, Junichi
	CreatorName Tateiwa, Daisuke
	NRID 1000050964334 1000050964334 ORCID 0000-0002-0747-8854 0000-0002-0747-8854 researchmap permalink yuichiro.ukon yuichiro.ukon CreatorName Ukon, Yuichiro
	CreatorName Zeynep, Bal
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
	NRID 1000070623982 1000070623982 ORCID 0000-0003-4882-2997 0000-0003-4882-2997 researchmap permalink 911_0 911_0 Scopus Author ID 25824322100 25824322100 CreatorName Kaito, Takashi
Subject	Bone marrow-derived stromal cells
	Magnetic field
	Mechanical strength
	Orientation intensity
	Osteoblastic differentiation
Description	Okada R., Yamato K., Kawakami M., et al. Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells. Bone Reports, 14, 100757. https://doi.org/10.1016/j.bonr.2021.100757.
Abstract	Effects of high magnetic fields [MFs, ≥ 1 T (T)] on osteoblastic differentiation and the orientation of cells or matrix proteins have been reported. However, the effect of low MFs (< 1 T) on the orientation of bone formation is not well known. This study was performed to verify the effects of low MFs on osteoblastic differentiation, bone formation, and orientation of both cells and newly formed bone. An apparatus was prepared with two magnets (190 mT) aligned in parallel to generate a parallel MF. In vitro, bone marrow-derived stromal cells of rats were used to assess the effects of low MFs on cell orientation, osteoblastic differentiation, and mineralization. A bone morphogenetic protein (BMP)-2-induced ectopic bone model was used to elucidate the effect of low MFs on microstructural indices, trabecula orientation, and the apatite c-axis orientation of newly formed bone. Low MFs resulted in an increased ratio of cells oriented perpendicular to the direction of the MF and promoted osteoblastic differentiation in vitro. Moreover, in vivo analysis demonstrated that low MFs promoted bone formation and changed the orientation of trabeculae and apatite crystal in a direction perpendicular to the MF. These changes led to an increase in the mechanical strength of rhBMP-2-induced bone. These results suggest that the application of low MFs has potential to facilitate the regeneration of bone with sufficient mechanical strength by controlling the orientation of newly formed bone.
Publisher	Elsevier
Source Title	Bone Reports
Volume (Issue)	14
Page	100757
Date of Issued	2021-06-01
Language	English
Handle URL	https://hdl.handle.net/11094/89784
eISSN	23521872
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bonr.2021.100757
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Bone Reports. 2021, 14, p. 100757
DC.title	Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells
DC.creator	Okada, Rintaro
	Yamato, Kai
	Kawakami, Minoru
	Kodama, Joe
	Kushioka, Junichi
	Tateiwa, Daisuke
	Ukon, Yuichiro
	Zeynep, Bal
	Ishimoto, Takuya
	Nakano, Takayoshi
	Yoshikawa, Hideki
	Kaito, Takashi
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-06-01
DC.identifier	https://doi.org/10.1016/j.bonr.2021.100757
DC.subject	Bone marrow-derived stromal cells
	Magnetic field
	Mechanical strength
	Orientation intensity
	Osteoblastic differentiation
DC.description	Okada R., Yamato K., Kawakami M., et al. Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells. Bone Reports, 14, 100757. https://doi.org/10.1016/j.bonr.2021.100757.
DCTERMS.abstract	Effects of high magnetic fields [MFs, ≥ 1 T (T)] on osteoblastic differentiation and the orientation of cells or matrix proteins have been reported. However, the effect of low MFs (< 1 T) on the orientation of bone formation is not well known. This study was performed to verify the effects of low MFs on osteoblastic differentiation, bone formation, and orientation of both cells and newly formed bone. An apparatus was prepared with two magnets (190 mT) aligned in parallel to generate a parallel MF. In vitro, bone marrow-derived stromal cells of rats were used to assess the effects of low MFs on cell orientation, osteoblastic differentiation, and mineralization. A bone morphogenetic protein (BMP)-2-induced ectopic bone model was used to elucidate the effect of low MFs on microstructural indices, trabecula orientation, and the apatite c-axis orientation of newly formed bone. Low MFs resulted in an increased ratio of cells oriented perpendicular to the direction of the MF and promoted osteoblastic differentiation in vitro. Moreover, in vivo analysis demonstrated that low MFs promoted bone formation and changed the orientation of trabeculae and apatite crystal in a direction perpendicular to the MF. These changes led to an increase in the mechanical strength of rhBMP-2-induced bone. These results suggest that the application of low MFs has potential to facilitate the regeneration of bone with sufficient mechanical strength by controlling the orientation of newly formed bone.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	89784
citation_title	Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells
citation_author	Okada, Rintaro
	Yamato, Kai
	Kawakami, Minoru
	Kodama, Joe
	Kushioka, Junichi
	Tateiwa, Daisuke
	Ukon, Yuichiro
	Zeynep, Bal
	Ishimoto, Takuya
	Nakano, Takayoshi
	Yoshikawa, Hideki
	Kaito, Takashi
citation_publisher	Elsevier
citation_language	English
citation_date	2021-06-01
citation_journal_title	Bone Reports
citation_volume	14
citation_firstpage	100757
citation_doi	https://doi.org/10.1016/j.bonr.2021.100757
citation_public_url	https://hdl.handle.net/11094/89784

Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells

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