Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

Okamura, Gensuke; Ebina, Kosuke; Hirao, Makoto; Chijimatsu, Ryota; Yonetani, Yasukazu; Etani, Yuki; Miyama, Akira; Takami, Kenji; Goshima, Atsushi; Yoshikawa, Hideki; Ishimoto, Takuya; Nakano, Takayoshi; Hamada, Masayuki; Kanamoto, Takashi; Nakata, Ken

doi:https://doi.org/10.3390/ijms22010300

Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

Okamura, Gensuke; Ebina, Kosuke; Hirao, Makoto et al.

International Journal of Molecular Sciences, 2020, 22(1)

Number of Access:1,467（2025-07-18 10:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89793

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Title	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
Creator	CreatorName Okamura, Gensuke
	CreatorName Ebina, Kosuke
	NRID 1000010527708 1000010527708 CreatorName Hirao, Makoto
	CreatorName Chijimatsu, Ryota
	NRID 1000080642090 1000080642090 CreatorName Yonetani, Yasukazu
	NRID 1000060898930 1000060898930 CreatorName Etani, Yuki
	CreatorName Miyama, Akira
	CreatorName Takami, Kenji
	CreatorName Goshima, Atsushi
	NRID 1000060191558 1000060191558 CreatorName Yoshikawa, Hideki
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	CreatorName Hamada, Masayuki
	NRID 1000020512049 1000020512049 researchmap permalink kanamoto kanamoto Scopus Author ID 7005012135 7005012135 CreatorName Kanamoto, Takashi
	NRID 1000000283747 1000000283747 researchmap permalink ken-nakata ken-nakata Scopus Author ID 7402151308 7402151308 CreatorName Nakata, Ken
Subject	Basic fibroblast growth factor
	Cartilage regenera-tion
	Synovial mesenchymal stem cells
	Xenograft
Description	Okamura G., Ebina K., Hirao M., et al. Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration. International Journal of Molecular Sciences, 22, 1, 1. https://doi.org/10.3390/ijms22010300.
Abstract	Synovial mesenchymal stem cell (SMSC) is the promising cell source of cartilage regeneration but has several issues to overcome such as limited cell proliferation and heterogeneity of cartilage regeneration ability. Previous reports demonstrated that basic fibroblast growth factor (bFGF) can promote proliferation and cartilage differentiation potential of MSCs in vitro, although no reports show its beneficial effect in vivo. The purpose of this study is to investigate the promoting effect of bFGF on cartilage regeneration using human SMSC in vivo. SMSCs were cultured with or without bFGF in a growth medium, and 2 × 105 cells were aggregated to form a synovial pellet. Synovial pellets were implanted into osteochondral defects induced in the femoral trochlea of severe combined immunodeficient mice, and histological evaluation was performed after eight weeks. The presence of implanted SMSCs was confirmed by the observation of human vimentin immunostaining-positive cells. Interestingly, broad lacunae structures and cartilage substrate stained by Safranin-O were observed only in the bFGF (+) group. The bFGF (+) group had significantly higher O’Driscoll scores in the cartilage repair than the bFGF (−) group. The addition of bFGF to SMSC growth culture may be a useful treatment option to promote cartilage regeneration in vivo.
Publisher	MDPI
Source Title	International Journal of Molecular Sciences
Volume (Issue)	22(1)
Date of Issued	2020-12-30
Language	English
Handle URL	https://hdl.handle.net/11094/89793
PISSN	16616596
eISSN	14220067
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.3390/ijms22010300
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	International Journal of Molecular Sciences. 2020, 22(1)
DC.title	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
DC.creator	Okamura, Gensuke
	Ebina, Kosuke
	Hirao, Makoto
	Chijimatsu, Ryota
	Yonetani, Yasukazu
	Etani, Yuki
	Miyama, Akira
	Takami, Kenji
	Goshima, Atsushi
	Yoshikawa, Hideki
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hamada, Masayuki
	Kanamoto, Takashi
	Nakata, Ken
DC.publisher	MDPI
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-12-30
DC.identifier	https://doi.org/10.3390/ijms22010300
DC.subject	Basic fibroblast growth factor
	Cartilage regenera-tion
	Synovial mesenchymal stem cells
	Xenograft
DC.description	Okamura G., Ebina K., Hirao M., et al. Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration. International Journal of Molecular Sciences, 22, 1, 1. https://doi.org/10.3390/ijms22010300.
DCTERMS.abstract	Synovial mesenchymal stem cell (SMSC) is the promising cell source of cartilage regeneration but has several issues to overcome such as limited cell proliferation and heterogeneity of cartilage regeneration ability. Previous reports demonstrated that basic fibroblast growth factor (bFGF) can promote proliferation and cartilage differentiation potential of MSCs in vitro, although no reports show its beneficial effect in vivo. The purpose of this study is to investigate the promoting effect of bFGF on cartilage regeneration using human SMSC in vivo. SMSCs were cultured with or without bFGF in a growth medium, and 2 × 105 cells were aggregated to form a synovial pellet. Synovial pellets were implanted into osteochondral defects induced in the femoral trochlea of severe combined immunodeficient mice, and histological evaluation was performed after eight weeks. The presence of implanted SMSCs was confirmed by the observation of human vimentin immunostaining-positive cells. Interestingly, broad lacunae structures and cartilage substrate stained by Safranin-O were observed only in the bFGF (+) group. The bFGF (+) group had significantly higher O’Driscoll scores in the cartilage repair than the bFGF (−) group. The addition of bFGF to SMSC growth culture may be a useful treatment option to promote cartilage regeneration in vivo.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89793
citation_title	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
citation_author	Okamura, Gensuke
	Ebina, Kosuke
	Hirao, Makoto
	Chijimatsu, Ryota
	Yonetani, Yasukazu
	Etani, Yuki
	Miyama, Akira
	Takami, Kenji
	Goshima, Atsushi
	Yoshikawa, Hideki
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hamada, Masayuki
	Kanamoto, Takashi
	Nakata, Ken
citation_publisher	MDPI
citation_language	English
citation_date	2020-12-30
citation_journal_title	International Journal of Molecular Sciences
citation_volume	22
citation_issue	1
citation_doi	https://doi.org/10.3390/ijms22010300
citation_public_url	https://hdl.handle.net/11094/89793

Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

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