Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass

Moriishi, Takeshi; Ozasa, Ryosuke; Ishimoto, Takuya; Nakano, Takayoshi; Hasegawa, Tomoka; Miyazaki, Toshihiro; Liu, Wenguang; Fukuyama, Ryo; Wang, Yuying; Komori, Hisato; Qin, Xin; Amizuka, Norio; Komori, Toshihisa

doi:https://doi.org/10.1371/journal.pgen.1008586

Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass

Moriishi, Takeshi; Ozasa, Ryosuke; Ishimoto, Takuya et al.

PLoS Genetics, 2020, 16(5), e1008586

Number of Access:835（2025-07-18 10:03 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89805

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Title	Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass
Creator	NRID 1000020380983 1000020380983 CreatorName Moriishi, Takeshi
	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	NRID 1000050739349 1000050739349 CreatorName Hasegawa, Tomoka
	NRID 1000010174161 1000010174161 CreatorName Miyazaki, Toshihiro
	CreatorName Liu, Wenguang
	NRID 1000020389117 1000020389117 CreatorName Fukuyama, Ryo
	CreatorName Wang, Yuying
	NRID 1000080770411 1000080770411 CreatorName Komori, Hisato
	CreatorName Qin, Xin
	NRID 1000030242431 1000030242431 CreatorName Amizuka, Norio
	CreatorName Komori, Toshihisa
Description	Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass. Takeshi Moriishi et al. PLOS Genetics. 2020. 5(28)doi.org/10.1371/journal.pgen.1008586
Abstract	The strength of bone depends on bone quantity and quality. Osteocalcin (Ocn) is the most abundant noncollagenous protein in bone and is produced by osteoblasts. It has been previously claimed that Ocn inhibits bone formation and also functions as a hormone to regulate insulin secretion in the pancreas, testosterone synthesis in the testes, and muscle mass. We generated Ocn-deficient (Ocn–/–) mice by deleting Bglap and Bglap2. Analysis of Ocn–/ –mice revealed that Ocn is not involved in the regulation of bone quantity, glucose metabolism, testosterone synthesis, or muscle mass. The orientation degree of collagen fibrils and size of biological apatite (BAp) crystallites in the c-axis were normal in the Ocn–/–bone. However, the crystallographic orientation of the BAp c-axis, which is normally parallel to collagen fibrils, was severely disrupted, resulting in reduced bone strength. These results demonstrate that Ocn is required for bone quality and strength by adjusting the alignment of BAp crystallites parallel to collagen fibrils; but it does not function as a hormone.
Publisher	Public Library of Science
Source Title	PLoS Genetics
Volume (Issue)	16(5)
Page	e1008586
Date of Issued	2020-05-28
Language	English
Handle URL	https://hdl.handle.net/11094/89805
PISSN	15537390
eISSN	15537404
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1371/journal.pgen.1008586
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	PLoS Genetics. 2020, 16(5), p. e1008586
DC.title	Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass
DC.creator	Moriishi, Takeshi
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hasegawa, Tomoka
	Miyazaki, Toshihiro
	Liu, Wenguang
	Fukuyama, Ryo
	Wang, Yuying
	Komori, Hisato
	Qin, Xin
	Amizuka, Norio
	Komori, Toshihisa
DC.publisher	Public Library of Science
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-05-28
DC.identifier	https://doi.org/10.1371/journal.pgen.1008586
DC.description	Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass. Takeshi Moriishi et al. PLOS Genetics. 2020. 5(28)doi.org/10.1371/journal.pgen.1008586
DCTERMS.abstract	The strength of bone depends on bone quantity and quality. Osteocalcin (Ocn) is the most abundant noncollagenous protein in bone and is produced by osteoblasts. It has been previously claimed that Ocn inhibits bone formation and also functions as a hormone to regulate insulin secretion in the pancreas, testosterone synthesis in the testes, and muscle mass. We generated Ocn-deficient (Ocn–/–) mice by deleting Bglap and Bglap2. Analysis of Ocn–/ –mice revealed that Ocn is not involved in the regulation of bone quantity, glucose metabolism, testosterone synthesis, or muscle mass. The orientation degree of collagen fibrils and size of biological apatite (BAp) crystallites in the c-axis were normal in the Ocn–/–bone. However, the crystallographic orientation of the BAp c-axis, which is normally parallel to collagen fibrils, was severely disrupted, resulting in reduced bone strength. These results demonstrate that Ocn is required for bone quality and strength by adjusting the alignment of BAp crystallites parallel to collagen fibrils; but it does not function as a hormone.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89805
citation_title	Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass
citation_author	Moriishi, Takeshi
	Ozasa, Ryosuke
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hasegawa, Tomoka
	Miyazaki, Toshihiro
	Liu, Wenguang
	Fukuyama, Ryo
	Wang, Yuying
	Komori, Hisato
	Qin, Xin
	Amizuka, Norio
	Komori, Toshihisa
citation_publisher	Public Library of Science
citation_language	English
citation_date	2020-05-28
citation_journal_title	PLoS Genetics
citation_volume	16
citation_issue	5
citation_firstpage	e1008586
citation_doi	https://doi.org/10.1371/journal.pgen.1008586
citation_public_url	https://hdl.handle.net/11094/89805

Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass

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