3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites

Matsugaki, Aira; Matsuzaka, Tadaaki; Murakami, Ami et al.

International Journal of Bioprinting, 2020, 6(4), 293

Number of Access:400（2025-07-27 15:52 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89810

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Title
3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites
Creator
NRID
1000010592529 1000010592529
ORCID
0000-0002-8427-7124 0000-0002-8427-7124
researchmap permalink
aira aira
Scopus Author ID
24172720900 24172720900
CreatorName
Matsugaki, Aira
CreatorName
Matsuzaka, Tadaaki
CreatorName
Murakami, Ami
CreatorName
Wang, Pan
NRID
1000030243182 1000030243182
ORCID
0000-0001-8052-1698 0000-0001-8052-1698
researchmap permalink
read0013987 read0013987
Scopus Author ID
7403896013 7403896013
CreatorName
Nakano, Takayoshi
Subject
Bioprinting
Collagen substrate
Mineralization
Osteocyte, 3D arrangement of bone matrix
3D arrangement of bone matrix
Description
Matsugaki A., Matsuzaka T., Murakami A., et al. 3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites. International Journal of Bioprinting, 6, 4, 293. https://doi.org/10.18063/IJB.V6I4.293.
Abstract
Although three-dimensional (3D) bioprinting techniques enable the construction of various living tissues and organs, the generation of bone-like oriented microstructures with anisotropic texture remains a challenge. Inside the mineralized bone matrix, osteocytes play mechanosensing roles in an ordered manner with a well-developed lacunar-canaliculi system. Therefore, control of cellular arrangement and dendritic processes is indispensable for construction of artificially controlled 3D bone-mimetic architecture. Herein, we propose an innovative methodology to induce controlled arrangement of osteocyte dendritic processes using the laminated layer method of oriented collagen sheets, combined with a custom-made fluid flow stimuli system. Osteocyte dendritic processes showed elongation depending on the competitive directional relationship between flow and substrate. To the best of our knowledge, this study is the first to report the successful construction of the anisotropic bone-mimetic microstructure and further demonstrate that the dendritic process formation in osteocytes can be controlled with selective fluid flow stimuli, specifically by regulating focal adhesion. Our results demonstrate how osteocytes adapt to mechanical stimuli by optimizing the anisotropic maturation of dendritic cell processes.
Publisher
Whioce Publishing Pte.
Source Title
International Journal of Bioprinting
Volume (Issue)
6(4)
Page
293
Date of Issued
2020-06-27
Language
English
Handle URL
https://hdl.handle.net/11094/89810
eISSN
24248002
Relation.isIdenticalTo
URI
https://ijb.sg/index.php/int-j-bioprinting/article/view/293
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article