Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly

Nakanishi, Yohei; Matsugaki, Aira; Kawahara, Kosuke; Ninomiya, Takafumi; Sawada, Hiroshi; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.biomaterials.2019.04.016

Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly

Nakanishi, Yohei; Matsugaki, Aira; Kawahara, Kosuke et al.

Biomaterials, 2019, 209, 103-110

Number of Access:584（2025-07-27 15:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89818

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Title	Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly
Creator	CreatorName Nakanishi, Yohei
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	CreatorName Kawahara, Kosuke
	CreatorName Ninomiya, Takafumi
	CreatorName Sawada, Hiroshi
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Bone matrix
	Cell orientation
	Focal adhesion
	Osteoblast
	Tspan11
Description	Nakanishi Y., Matsugaki A., Kawahara K., et al. Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly. Biomaterials, 209, 103. https://doi.org/10.1016/j.biomaterials.2019.04.016.
Abstract	During tissue construction, cells coordinate extracellular matrix (ECM)assembly depending on the cellular arrangement. The traditional understanding of the relationship between the ECM and cells is limited to the orientation-matched interaction between them. Indeed, it is commonly accepted that the bone matrix (collagen/apatite)is formed along osteoblast orientation. Nonetheless, our recent findings are contrary to the above theory; osteoblasts on nanogrooves organize formation of the bone matrix perpendicular to cell orientation. However, the precise molecular mechanisms underlying the orthogonal organization of bone matrix are still unknown. Here, we show that mature fibrillar focal adhesions (FAs)facilitate the perpendicular arrangement between cells and bone matrix. The osteoblasts aligned along nanogrooves expressed highly mature fibrillar FAs mediated by integrin clustering. Microarray analysis revealed that Tspan11, a member of the transmembrane tetraspanin protein family, was upregulated in cells on the nanogrooved surface compared with that in cells on isotropic, flat, or rough surfaces. Tspan11 silencing significantly disrupted osteoblast alignment and further construction of aligned bone matrix orthogonal to cell orientation. Our results demonstrate that the unique bone matrix formation orthogonal to cell alignment is facilitated by FA maturation. To the best of our knowledge, this report is the first to show that FA assembly mediated by Tspan11 determines the direction of bone matrix organization.
Publisher	Elsevier
Source Title	Biomaterials
Volume (Issue)	209
Page	103-110
Date of Issued	2019-07-01
Language	English
Handle URL	https://hdl.handle.net/11094/89818
PISSN	01429612
eISSN	18785905
NCID	AA00110092
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.biomaterials.2019.04.016
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biomaterials. 2019, 209, p. 103-110
DC.title	Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly
DC.creator	Nakanishi, Yohei
	Matsugaki, Aira
	Kawahara, Kosuke
	Ninomiya, Takafumi
	Sawada, Hiroshi
	Nakano, Takayoshi
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-07-01
DC.identifier	https://doi.org/10.1016/j.biomaterials.2019.04.016
DC.subject	Bone matrix
	Cell orientation
	Focal adhesion
	Osteoblast
	Tspan11
DC.description	Nakanishi Y., Matsugaki A., Kawahara K., et al. Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly. Biomaterials, 209, 103. https://doi.org/10.1016/j.biomaterials.2019.04.016.
DCTERMS.abstract	During tissue construction, cells coordinate extracellular matrix (ECM)assembly depending on the cellular arrangement. The traditional understanding of the relationship between the ECM and cells is limited to the orientation-matched interaction between them. Indeed, it is commonly accepted that the bone matrix (collagen/apatite)is formed along osteoblast orientation. Nonetheless, our recent findings are contrary to the above theory; osteoblasts on nanogrooves organize formation of the bone matrix perpendicular to cell orientation. However, the precise molecular mechanisms underlying the orthogonal organization of bone matrix are still unknown. Here, we show that mature fibrillar focal adhesions (FAs)facilitate the perpendicular arrangement between cells and bone matrix. The osteoblasts aligned along nanogrooves expressed highly mature fibrillar FAs mediated by integrin clustering. Microarray analysis revealed that Tspan11, a member of the transmembrane tetraspanin protein family, was upregulated in cells on the nanogrooved surface compared with that in cells on isotropic, flat, or rough surfaces. Tspan11 silencing significantly disrupted osteoblast alignment and further construction of aligned bone matrix orthogonal to cell orientation. Our results demonstrate that the unique bone matrix formation orthogonal to cell alignment is facilitated by FA maturation. To the best of our knowledge, this report is the first to show that FA assembly mediated by Tspan11 determines the direction of bone matrix organization.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89818
citation_title	Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly
citation_author	Nakanishi, Yohei
	Matsugaki, Aira
	Kawahara, Kosuke
	Ninomiya, Takafumi
	Sawada, Hiroshi
	Nakano, Takayoshi
citation_publisher	Elsevier
citation_language	English
citation_date	2019-07-01
citation_journal_title	Biomaterials
citation_volume	209
citation_firstpage	103
citation_lastpage	110
citation_doi	https://doi.org/10.1016/j.biomaterials.2019.04.016
citation_public_url	https://hdl.handle.net/11094/89818

Unique arrangement of bone matrix orthogonal to osteoblast alignment controlled by Tspan11-mediated focal adhesion assembly

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