Biomimetic mineralization using matrix vesicle nanofragments

Kunitomi, Yosuke; Hara, Emilio Satoshi; Okada, Masahiro; Nagaoka, Noriyuki; Kuboki, Takuo; Nakano, Takayoshi; Kamioka, Hiroshi; Matsumoto, Takuya

doi:https://doi.org/10.1002/jbm.a.36618

Biomimetic mineralization using matrix vesicle nanofragments

Kunitomi, Yosuke; Hara, Emilio Satoshi; Okada, Masahiro et al.

Journal of Biomedical Materials Research - Part A, 2019, 107(5), 1021-1030

Number of Access:1,001（2025-08-21 04:35 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89820

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Title	Biomimetic mineralization using matrix vesicle nanofragments
Creator	CreatorName Kunitomi, Yosuke
	NRID 1000040779443 1000040779443 CreatorName Hara, Emilio Satoshi
	NRID 1000070416220 1000070416220 CreatorName Okada, Masahiro
	NRID 1000070304326 1000070304326 CreatorName Nagaoka, Noriyuki
	NRID 1000000225195 1000000225195 CreatorName Kuboki, Takuo
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	NRID 1000080253219 1000080253219 CreatorName Kamioka, Hiroshi
	NRID 1000040324793 1000040324793 CreatorName Matsumoto, Takuya
Description	Kunitomi Y., Hara E.S., Okada M., et al. Biomimetic mineralization using matrix vesicle nanofragments. Journal of Biomedical Materials Research - Part A, 107, 5, 1021. https://doi.org/10.1002/jbm.a.36618.
Abstract	In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021–1030, 2019.
Publisher	John Wiley and Sons
Source Title	Journal of Biomedical Materials Research - Part A
Volume (Issue)	107(5)
Page	1021-1030
Date of Issued	2019-01-24
Language	English
Handle URL	https://hdl.handle.net/11094/89820
PISSN	15493296
eISSN	15524965
NCID	AA11881516
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1002/jbm.a.36618
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biomedical Materials Research - Part A. 2019, 107(5), p. 1021-1030
DC.title	Biomimetic mineralization using matrix vesicle nanofragments
DC.creator	Kunitomi, Yosuke
	Hara, Emilio Satoshi
	Okada, Masahiro
	Nagaoka, Noriyuki
	Kuboki, Takuo
	Nakano, Takayoshi
	Kamioka, Hiroshi
	Matsumoto, Takuya
DC.publisher	John Wiley and Sons
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-01-24
DC.identifier	https://doi.org/10.1002/jbm.a.36618
DC.description	Kunitomi Y., Hara E.S., Okada M., et al. Biomimetic mineralization using matrix vesicle nanofragments. Journal of Biomedical Materials Research - Part A, 107, 5, 1021. https://doi.org/10.1002/jbm.a.36618.
DCTERMS.abstract	In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021–1030, 2019.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	89820
citation_title	Biomimetic mineralization using matrix vesicle nanofragments
citation_author	Kunitomi, Yosuke
	Hara, Emilio Satoshi
	Okada, Masahiro
	Nagaoka, Noriyuki
	Kuboki, Takuo
	Nakano, Takayoshi
	Kamioka, Hiroshi
	Matsumoto, Takuya
citation_publisher	John Wiley and Sons
citation_language	English
citation_date	2019-01-24
citation_journal_title	Journal of Biomedical Materials Research - Part A
citation_volume	107
citation_issue	5
citation_firstpage	1021
citation_lastpage	1030
citation_doi	https://doi.org/10.1002/jbm.a.36618
citation_public_url	https://hdl.handle.net/11094/89820

Biomimetic mineralization using matrix vesicle nanofragments

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