Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

Ozasa, Ryosuke; Matsugaki, Aira; Isobe, Yoshihiro; Saku, Taro; Yun, Hui Suk; Nakano, Takayoshi

doi:https://doi.org/10.1002/jbm.a.36238

Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

Ozasa, Ryosuke; Matsugaki, Aira; Isobe, Yoshihiro et al.

Journal of Biomedical Materials Research - Part A, 2017, 106(2), 360-369

Number of Access:722（2025-07-27 15:50 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89833

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Title	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
Creator	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	CreatorName Isobe, Yoshihiro
	CreatorName Saku, Taro
	CreatorName Yun, Hui Suk
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Description	Ozasa R., Matsugaki A., Isobe Y., et al. Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model. Journal of Biomedical Materials Research - Part A, 106, 2, 360. https://doi.org/10.1002/jbm.a.36238.
Abstract	Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold. The oriented collagen scaffold allowed hiPSCs-derived osteoblast alignment and further construction of anisotropic bone matrix which mimics the bone tissue microstructure. To the best of our knowledge, this is the first report showing the construction of bone mimetic anisotropic bone matrix microstructure from hiPSCs. Moreover, we demonstrated for the first time that the hiPSCs-derived osteoblasts possess a high level of intact functionality to regulate cell alignment. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 360–369, 2018.
Publisher	John Wiley and Sons
Source Title	Journal of Biomedical Materials Research - Part A
Volume (Issue)	106(2)
Page	360-369
Date of Issued	2017-09-16
Language	English
Handle URL	https://hdl.handle.net/11094/89833
PISSN	15493296
eISSN	15524965
NCID	AA11881516
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1002/jbm.a.36238
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biomedical Materials Research - Part A. 2017, 106(2), p. 360-369
DC.title	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
DC.creator	Ozasa, Ryosuke
	Matsugaki, Aira
	Isobe, Yoshihiro
	Saku, Taro
	Yun, Hui Suk
	Nakano, Takayoshi
DC.publisher	John Wiley and Sons
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-09-16
DC.identifier	https://doi.org/10.1002/jbm.a.36238
DC.description	Ozasa R., Matsugaki A., Isobe Y., et al. Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model. Journal of Biomedical Materials Research - Part A, 106, 2, 360. https://doi.org/10.1002/jbm.a.36238.
DCTERMS.abstract	Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold. The oriented collagen scaffold allowed hiPSCs-derived osteoblast alignment and further construction of anisotropic bone matrix which mimics the bone tissue microstructure. To the best of our knowledge, this is the first report showing the construction of bone mimetic anisotropic bone matrix microstructure from hiPSCs. Moreover, we demonstrated for the first time that the hiPSCs-derived osteoblasts possess a high level of intact functionality to regulate cell alignment. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 360–369, 2018.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	89833
citation_title	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
citation_author	Ozasa, Ryosuke
	Matsugaki, Aira
	Isobe, Yoshihiro
	Saku, Taro
	Yun, Hui Suk
	Nakano, Takayoshi
citation_publisher	John Wiley and Sons
citation_language	English
citation_date	2017-09-16
citation_journal_title	Journal of Biomedical Materials Research - Part A
citation_volume	106
citation_issue	2
citation_firstpage	360
citation_lastpage	369
citation_doi	https://doi.org/10.1002/jbm.a.36238
citation_public_url	https://hdl.handle.net/11094/89833

Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

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