Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu

Hara, Emilio Satoshi; Okada, Masahiro; Kuboki, Takuo et al.

Journal of Materials Chemistry B, 2018, 6(38), 6153-6161

Number of Access:699（2025-08-21 04:36 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89834

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Title
Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu
Creator
NRID
1000040779443 1000040779443
CreatorName
Hara, Emilio Satoshi
NRID
1000070416220 1000070416220
CreatorName
Okada, Masahiro
NRID
1000000225195 1000000225195
CreatorName
Kuboki, Takuo
NRID
1000030243182 1000030243182
ORCID
0000-0001-8052-1698 0000-0001-8052-1698
researchmap permalink
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Scopus Author ID
7403896013 7403896013
CreatorName
Nakano, Takayoshi
NRID
1000040324793 1000040324793
CreatorName
Matsumoto, Takuya
Description
Hara E.S., Okada M., Kuboki T., et al. Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu. Journal of Materials Chemistry B, 6, 38, 6153. https://doi.org/10.1039/C8TB01544A.
Abstract
Bone is a sophisticated organic-inorganic hybrid material, whose formation involves a complex spatio-temporal sequence of events regulated by the cells. A deeper understanding of the mechanisms behind bone mineralization at different size scales, and using a multidisciplinary approach, may uncover novel pathways for the design and fabrication of functional bone tissue in vitro. The objectives of this study were first to investigate the environmental factors that prime initial mineralization using the secondary ossification center as an in vivo model, and then to apply the obtained knowledge for rapid in vitro synthesis of bone-like tissue. First, the direct and robust measurement of pH showed that femur epiphysis is alkaline (pH ≅ 8.5) at the initial mineral stage at post-natal day 6. We showed that the alkaline milieu is decisive not only for alkaline phosphatase activity, which precedes mineral formation at P6, but also for determining initial mineral precipitation and spherical morphology. Next, engineering approaches were used to synthesize bone-like tissue based on alkaline milieu and artificial chondrocyte membrane nanofragments, previously shown to be the nucleation site for mineral formation. Interestingly, mineralization using artificial cell membrane nanofragments was achieved in just 1 day. Finally, ex vivo culture of femur epiphysis in alkaline pH strongly induced chondrocyte burst, which was previously shown to be the origin of chondrocyte membrane nanofragments, and also enhanced mineral formation. Taken together, these findings not only shed more light on the microenvironmental conditions that prime initial bone formation in vivo, but they also show that alkaline milieu can be used as an important factor for enhancing methods for in vitro synthesis of bone tissue.
Publisher
Royal Society of Chemistry
Source Title
Journal of Materials Chemistry B
Volume (Issue)
6(38)
Page
6153-6161
Date of Issued
2018-09-11
Language
English
Handle URL
https://hdl.handle.net/11094/89834
PISSN
20507518
eISSN
2050750X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1039/C8TB01544A
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article