Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur

Ozasa, Ryosuke; Nakatsu, Mayuko; Moriguchi, Atsushi; Sasaki, Kyohei; Ishimoto, Takuya; Okada, Masahiro; Matsumoto, Takuya; Nakano, Takayoshi

doi:https://doi.org/10.2320/matertrans.MT-M2019341

Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur

Ozasa, Ryosuke; Nakatsu, Mayuko; Moriguchi, Atsushi et al.

Materials Transactions, 2020, 61(2), 381-386

Number of Access:1,014（2025-07-18 09:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89896

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Title	Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur
Creator	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 CreatorName Ozasa, Ryosuke
	CreatorName Nakatsu, Mayuko
	NRID 1000010273666 1000010273666 CreatorName Moriguchi, Atsushi
	CreatorName Sasaki, Kyohei
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000070416220 1000070416220 CreatorName Okada, Masahiro
	NRID 1000040324793 1000040324793 CreatorName Matsumoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Apatite c-axis orientation
	Bone regeneration
	Collagen orientation
Description	Ozasa R., Nakatsu M., Moriguchi A., et al. Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur. Materials Transactions 61, 381 (2020); https://doi.org/10.2320/matertrans.MT-M2019341.
Abstract	In this study, we focused on the preferential orientation of the extracellular matrix (ECM) of bone, since ECM orientation has been shown to significantly affect the mechanical functions of bones. Bone analysis is in most cases based on the premise that the apatite crystallizes on the collagen template such that its c-axis is parallel with the running direction of the collagen fibril. Bone regeneration analysis has also been discussed assuming that the apatite c-axis orientation reflects collagen orientation. To understand the regeneration processes of both collagen and apatite individually, the preferential orientations of apatite and collagen in regenerated bone were simultaneously analyzed using a bone regeneration model of mouse femur with an 0.8-mm drill hole defect. The defects in mouse femur were filled with mineralized bone matrix, which shows an intact mineral density. However, the directions of orientation of the collagen and apatite deviate from the femur longitudinal axis in the regenerated bone. Moreover, electron diffraction analysis revealed that the apatite c-axis aligned along the extended axis of a collagen fibril both in regenerated and intact bones, indicating that the direction of the apatite c-axis is regulated by collagen fibril orientation even in the regenerated bone. In conclusion, the less-oriented apatite crystallite observed in the regenerated bone was shown to be formed due to the less-oriented collagen fibrils.
Publisher	The Japan Institute of Metals and Materials
Source Title	Materials Transactions
Volume (Issue)	61(2)
Page	381-386
Date of Issued	2020-02-01
Language	English
Handle URL	https://hdl.handle.net/11094/89896
PISSN	13459678
eISSN	13475320
NCID	AA1151294X
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.2320/matertrans.MT-M2019341
Access Rights	open access
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Materials Transactions. 2020, 61(2), p. 381-386
DC.title	Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur
DC.creator	Ozasa, Ryosuke
	Nakatsu, Mayuko
	Moriguchi, Atsushi
	Sasaki, Kyohei
	Ishimoto, Takuya
	Okada, Masahiro
	Matsumoto, Takuya
	Nakano, Takayoshi
DC.publisher	The Japan Institute of Metals and Materials
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-02-01
DC.identifier	https://doi.org/10.2320/matertrans.MT-M2019341
DC.subject	Apatite c-axis orientation
	Bone regeneration
	Collagen orientation
DC.description	Ozasa R., Nakatsu M., Moriguchi A., et al. Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur. Materials Transactions 61, 381 (2020); https://doi.org/10.2320/matertrans.MT-M2019341.
DCTERMS.abstract	In this study, we focused on the preferential orientation of the extracellular matrix (ECM) of bone, since ECM orientation has been shown to significantly affect the mechanical functions of bones. Bone analysis is in most cases based on the premise that the apatite crystallizes on the collagen template such that its c-axis is parallel with the running direction of the collagen fibril. Bone regeneration analysis has also been discussed assuming that the apatite c-axis orientation reflects collagen orientation. To understand the regeneration processes of both collagen and apatite individually, the preferential orientations of apatite and collagen in regenerated bone were simultaneously analyzed using a bone regeneration model of mouse femur with an 0.8-mm drill hole defect. The defects in mouse femur were filled with mineralized bone matrix, which shows an intact mineral density. However, the directions of orientation of the collagen and apatite deviate from the femur longitudinal axis in the regenerated bone. Moreover, electron diffraction analysis revealed that the apatite c-axis aligned along the extended axis of a collagen fibril both in regenerated and intact bones, indicating that the direction of the apatite c-axis is regulated by collagen fibril orientation even in the regenerated bone. In conclusion, the less-oriented apatite crystallite observed in the regenerated bone was shown to be formed due to the less-oriented collagen fibrils.
DC.format	89896
citation_title	Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur
citation_author	Ozasa, Ryosuke
	Nakatsu, Mayuko
	Moriguchi, Atsushi
	Sasaki, Kyohei
	Ishimoto, Takuya
	Okada, Masahiro
	Matsumoto, Takuya
	Nakano, Takayoshi
citation_publisher	The Japan Institute of Metals and Materials
citation_language	English
citation_date	2020-02-01
citation_journal_title	Materials Transactions
citation_volume	61
citation_issue	2
citation_firstpage	381
citation_lastpage	386
citation_doi	https://doi.org/10.2320/matertrans.MT-M2019341
citation_public_url	https://hdl.handle.net/11094/89896

Analysis of bone regeneration based on the relationship between the orientations of collagen and apatite in mouse femur

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