Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite

Gokcekaya, Ozkan; Ergun, Celaletdin; Webster, Thomas J.; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.ceramint.2022.10.232

Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite

Gokcekaya, Ozkan; Ergun, Celaletdin; Webster, Thomas J. et al.

Ceramics International, 2022, 49(5), 7506-7514

Number of Access:449（2025-07-16 15:19 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/89905

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Title	Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite
Creator	CreatorName Gokcekaya, Ozkan
	CreatorName Ergun, Celaletdin
	CreatorName Webster, Thomas J.
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Borate
	Chemical synthesis
	Hydroxyapatite
	Incorporation
	Osteoblast adhesion
	Osteoblast spreading
Description	Gokcekaya O., Ergun C., Webster T.J., et al. Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite. Ceramics International; https://doi.org/https://doi.org/10.1016/j.ceramint.2022.10.232.
Abstract	The biological properties of hydroxyapatite (HA) are significantly influenced by its compositional characteristics especially doping elements and/or Ca/P ratio, which can be altered by precursor chemistry. In this study, a group of boronated (B-incorporated) hydroxyapatite (BHA) was synthesized using a precipitation method by setting the Ca/P ratio to the stoichiometric value of HA (1.67), while altering the precursor chemistry by adjusting either (Ca + B)/P (Ca-deficient precursor, BC) or Ca/(P + B) (P-deficient precursor, BP). After heat-treatment, the partial decomposition of the BC was observed, forming tricalcium phosphate as the byproduct, however, the BP showed phase stability at all temperatures. The B-ionic species in the form of (BO₂)⁻ and (BO₃)³⁻ were incorporated into the HA structure at the (PO₄)³⁻ and (OH)⁻ positions, respectively. The incorporation of the B species also facilitated the incorporation of (CO₃)²⁻ groups specifically in the BPs. This is the first finding on BHA reporting that preferential (CO₃)²⁻ incorporation depends on the precursor chemistry used. As a result, osteoblast adhesion was superior on the BPs compared to pure HA owing to the carbonated structure, increasing cell spreading area. As such, this in vitro study highlighted that the present P-deficient precursor approach for synthesizing BHA improved biocompatibility properties and should, thus, be further considered for the next-generation of improved orthopedic applications.
Publisher	Elsevier
Source Title	Ceramics International
Volume (Issue)	49(5)
Page	7506-7514
Date of Issued	2022-10-20
Language	English
Handle URL	https://hdl.handle.net/11094/89905
PISSN	02728842
NCID	AA10622625
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1016/j.ceramint.2022.10.232
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Ceramics International. 2022, 49(5), p. 7506-7514
DC.title	Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite
DC.creator	Gokcekaya, Ozkan
	Ergun, Celaletdin
	Webster, Thomas J.
	Nakano, Takayoshi
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-10-20
DC.identifier	https://doi.org/10.1016/j.ceramint.2022.10.232
DC.subject	Borate
	Chemical synthesis
	Hydroxyapatite
	Incorporation
	Osteoblast adhesion
	Osteoblast spreading
DC.description	Gokcekaya O., Ergun C., Webster T.J., et al. Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite. Ceramics International; https://doi.org/https://doi.org/10.1016/j.ceramint.2022.10.232.
DCTERMS.abstract	The biological properties of hydroxyapatite (HA) are significantly influenced by its compositional characteristics especially doping elements and/or Ca/P ratio, which can be altered by precursor chemistry. In this study, a group of boronated (B-incorporated) hydroxyapatite (BHA) was synthesized using a precipitation method by setting the Ca/P ratio to the stoichiometric value of HA (1.67), while altering the precursor chemistry by adjusting either (Ca + B)/P (Ca-deficient precursor, BC) or Ca/(P + B) (P-deficient precursor, BP). After heat-treatment, the partial decomposition of the BC was observed, forming tricalcium phosphate as the byproduct, however, the BP showed phase stability at all temperatures. The B-ionic species in the form of (BO₂)⁻ and (BO₃)³⁻ were incorporated into the HA structure at the (PO₄)³⁻ and (OH)⁻ positions, respectively. The incorporation of the B species also facilitated the incorporation of (CO₃)²⁻ groups specifically in the BPs. This is the first finding on BHA reporting that preferential (CO₃)²⁻ incorporation depends on the precursor chemistry used. As a result, osteoblast adhesion was superior on the BPs compared to pure HA owing to the carbonated structure, increasing cell spreading area. As such, this in vitro study highlighted that the present P-deficient precursor approach for synthesizing BHA improved biocompatibility properties and should, thus, be further considered for the next-generation of improved orthopedic applications.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89905
citation_title	Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite
citation_author	Gokcekaya, Ozkan
	Ergun, Celaletdin
	Webster, Thomas J.
	Nakano, Takayoshi
citation_publisher	Elsevier
citation_language	English
citation_date	2022-10-20
citation_journal_title	Ceramics International
citation_volume	49
citation_issue	5
citation_firstpage	7506
citation_lastpage	7514
citation_doi	https://doi.org/10.1016/j.ceramint.2022.10.232
citation_public_url	https://hdl.handle.net/11094/89905

Influence of precursor deficiency sites for borate incorporation on the structural and biological properties of boronated hydroxyapatite

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