Chitin- and Streptavidin- Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery

Kato, Shunsuke; Takeuchi, Koki; Iwaki, Motonao et al.

Angewandte Chemie International Edition, 2023, 145, 8285

Number of Access:997（2025-08-15 11:00 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/91737

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
AngewChemIntEd_145_8285 pdf None 723 KB 88 2024.06.26  

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Chitin- and Streptavidin- Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery
Creator
NRID
1000060909125 1000060909125
researchmap permalink
s_kato_chem s_kato_chem
Scopus Author ID
57195564115 57195564115
CreatorName
Kato, Shunsuke
CreatorName
Takeuchi, Koki
CreatorName
Iwaki, Motonao
NRID
1000060344123 1000060344123
ORCID
0000-0002-4349-566X 0000-0002-4349-566X
researchmap permalink
myzkkntr myzkkntr
CreatorName
Miyazaki, Kentaro
NRID
1000090403162 1000090403162
ORCID
0000-0001-9069-7574 0000-0001-9069-7574
researchmap permalink
read0070175 read0070175
Scopus Author ID
7401678792 7401678792
CreatorName
Honda, Kohsuke
NRID
1000020222226 1000020222226
ORCID
0000-0002-2215-935X 0000-0002-2215-935X
researchmap permalink
20190602_Sun 20190602_Sun
Scopus Author ID
55735983400 55735983400
CreatorName
Hayashi, Takashi
Subject
Chitin
Cyclopropanation
Enzyme Screening
Hemoproteins
Protein Purification
Description
This is the peer reviewed version of the following article: Kato Shunsuke, Takeuchi Koki, Iwaki Motonao, et al., "Chitin- and Streptavidin- Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery", Angewandte Chemie International Edition, 26, June, 2023, which has been published in final form at https://doi.org/10.1002/anie.202303764. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Abstract
Affinity purification of recombinant proteins is an essential technique in biotechnology. However, current affinity purification methods are very cost-intensive, and this imposes limits on versatile use of affinity purification for obtaining purified proteins for a variety of applications. To overcome this problem, we developed a new affinity purification system which we call CSAP (chitin- and streptavidin-mediated affinity purification) for low-cost purification of Strep-tag II fusion proteins. The CSAP system is designed to utilize commercially available chitin powder as a chromatography matrix, thereby significantly improving the cost-efficiency of protein affinity purification. We investigated the CSAP system for protein screening in 96-well format as a demonstration. Through the screening of 96 types of purified hemoproteins, several proteins capable of the catalytic diastereodivergent synthesis of cyclopropanes were identified as candidates for an abiotic carbene transfer reaction.
Publisher
Wiley
Source Title
Angewandte Chemie International Edition
Volume (Issue)
145
Page
8285
Date of Issued
2023-06-26
Language
English
Handle URL
https://hdl.handle.net/11094/91737
PISSN
14337851
eISSN
15213773
NCID
AA0052535X
Relation.isVersionOf
DOI (Publisher Version)
https://doi.org/10.1002/anie.202303764
Access Rights
open access
CopyRight
© 2023 Wiley-VCH GmbH
oaire:version
Accepted Manuscript
Category
学術雑誌論文 Journal Article