Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

Hirano, Yasuhiro; Kinugasa, Yasuha; Kubota, Yoshino; Obuse, Chikashi; Haraguchi, Tokuko; Hiraoka, Yasushi

doi:https://doi.org/10.1093/jb/mvad017

Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

Hirano, Yasuhiro; Kinugasa, Yasuha; Kubota, Yoshino et al.

Journal of Biochemistry, 2023, 174(1), 33-46

Number of Access:840（2025-11-01 15:15 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/92448

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
JBiochem_174_1_33	pdf	None	1.79 MB	124	2024.02.17

Creative Commons : Attribution - NonCommercial - NoDerivatives

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
Creator	NRID 1000010508641 1000010508641 ORCID 0000-0001-7339-4053 0000-0001-7339-4053 researchmap permalink yasuhiro_hirano yasuhiro_hirano Scopus Author ID 57189010609 57189010609 CreatorName Hirano, Yasuhiro
	NRID 1000060852118 1000060852118 CreatorName Kinugasa, Yasuha
	researchmap permalink yoshinokubota yoshinokubota CreatorName Kubota, Yoshino
	NRID 1000000273855 1000000273855 researchmap permalink obuse obuse Scopus Author ID 6701730728 6701730728 CreatorName Obuse, Chikashi
	NRID 1000020359079 1000020359079 ORCID 0000-0002-3813-6785 0000-0002-3813-6785 researchmap permalink haraguchitokuko haraguchitokuko Scopus Author ID 7103088325 7103088325 CreatorName Haraguchi, Tokuko
	Scopus Author ID 7101710881 7101710881 CreatorName Hiraoka, Yasushi
Subject	Bqt4
	Lem2
	nuclear envelope
	proteome analysis
	Schizosaccharomyces pombe
Description	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record, Hirano Y., Kinugasa Y., Kubota Y., et al. Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast. 174(1), 33-46, (2023); is available online at: https://academic.oup.com/jb/article/174/1/33/7043862 and https://doi.org/10.1093/jb/mvad017
Abstract	The nuclear envelope (NE) is a double-membrane structure consisting of inner and outer membranes that spatially separate the nucleus from the cytoplasm, and its function is critical for cellular functions such as genome maintenance. In the fission yeast, Schizosaccharomyces pombe, the inner nuclear membrane proteins, Lem2 and Bqt4, play pivotal roles in maintaining the NE structure. We previously found that the double deletion of lem2+ and bqt4+ causes a synthetic lethal defect associated with severe NE rupture, and overexpression of Elo2, a solo very-long-chain fatty acid elongase, suppresses this defect by restoring the NE. However, the molecular basis of this restoration remains elusive. To address this, we identified Lem2- and Bqt4-binding proteins via immunoprecipitation and mass spectrometry in this study. Forty-five and 23 proteins were identified as Lem2- and Bqt4-binding proteins, respectively. Although these binding proteins partially overlapped, Lem2 and Bqt4 interacted with different types of lipid metabolic enzymes: Cho2, Ole1 and Erg11 for Lem2 and Cwh43 for Bqt4. These enzymes are known to be involved in various lipid synthesis processes, suggesting that Lem2 and Bqt4 may contribute to the regulation of lipid synthesis by binding to these enzymes.
Publisher	Oxford University Press
Source Title	Journal of Biochemistry
Volume (Issue)	174(1)
Page	33-46
Date of Issued	2023-07
Language	English
Handle URL	https://hdl.handle.net/11094/92448
PISSN	0021924X
eISSN	17562651
NCID	AA00694073
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1093/jb/mvad017
Access Rights	open access
CopyRight	© 2023 Oxford University Press. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Biochemistry. 2023, 174(1), p. 33-46
DC.title	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
DC.creator	Hirano, Yasuhiro
	Kinugasa, Yasuha
	Kubota, Yoshino
	Obuse, Chikashi
	Haraguchi, Tokuko
	Hiraoka, Yasushi
DC.publisher	Oxford University Press
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-07
DC.identifier	https://doi.org/10.1093/jb/mvad017
DC.subject	Bqt4
	Lem2
	nuclear envelope
	proteome analysis
	Schizosaccharomyces pombe
DC.description	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record, Hirano Y., Kinugasa Y., Kubota Y., et al. Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast. 174(1), 33-46, (2023); is available online at: https://academic.oup.com/jb/article/174/1/33/7043862 and https://doi.org/10.1093/jb/mvad017
DCTERMS.abstract	The nuclear envelope (NE) is a double-membrane structure consisting of inner and outer membranes that spatially separate the nucleus from the cytoplasm, and its function is critical for cellular functions such as genome maintenance. In the fission yeast, Schizosaccharomyces pombe, the inner nuclear membrane proteins, Lem2 and Bqt4, play pivotal roles in maintaining the NE structure. We previously found that the double deletion of lem2+ and bqt4+ causes a synthetic lethal defect associated with severe NE rupture, and overexpression of Elo2, a solo very-long-chain fatty acid elongase, suppresses this defect by restoring the NE. However, the molecular basis of this restoration remains elusive. To address this, we identified Lem2- and Bqt4-binding proteins via immunoprecipitation and mass spectrometry in this study. Forty-five and 23 proteins were identified as Lem2- and Bqt4-binding proteins, respectively. Although these binding proteins partially overlapped, Lem2 and Bqt4 interacted with different types of lipid metabolic enzymes: Cho2, Ole1 and Erg11 for Lem2 and Cwh43 for Bqt4. These enzymes are known to be involved in various lipid synthesis processes, suggesting that Lem2 and Bqt4 may contribute to the regulation of lipid synthesis by binding to these enzymes.
DC.rights	© 2023 Oxford University Press. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
DC.format	92448
citation_title	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
citation_author	Hirano, Yasuhiro
	Kinugasa, Yasuha
	Kubota, Yoshino
	Obuse, Chikashi
	Haraguchi, Tokuko
	Hiraoka, Yasushi
citation_publisher	Oxford University Press
citation_language	English
citation_date	2023-07
citation_journal_title	Journal of Biochemistry
citation_volume	174
citation_issue	1
citation_firstpage	33
citation_lastpage	46
citation_doi	https://doi.org/10.1093/jb/mvad017
citation_public_url	https://hdl.handle.net/11094/92448

Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

Link to primary information

Item Information

Show details