Centromere/kinetochore is assembled through CENP-C oligomerization

Hara, Masatoshi; Ariyoshi, Mariko; Sano, Tomoki et al.

Molecular Cell, 2023, 83(13), 2188-2205

Number of Access:766（2025-07-27 19:25 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/92473

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Title
Centromere/kinetochore is assembled through CENP-C oligomerization
Creator
NRID
1000030565099 1000030565099
ORCID
0000-0001-8433-1111 0000-0001-8433-1111
researchmap permalink
masatoshi_hara masatoshi_hara
Scopus Author ID
56069726900 56069726900
CreatorName
Hara, Masatoshi
NRID
1000080437243 1000080437243
ORCID
0000-0002-1361-2642 0000-0002-1361-2642
researchmap permalink
7000010594 7000010594
Scopus Author ID
7006547610 7006547610
CreatorName
Ariyoshi, Mariko
CreatorName
Sano, Tomoki
NRID
1000070868710 1000070868710
CreatorName
Nozawa, Ryu-Suke
NRID
1000060547058 1000060547058
CreatorName
Shinkai, Soya
NRID
1000050348843 1000050348843
CreatorName
Onami, Shuichi
CreatorName
Jansen, Isabelle
NRID
1000050421368 1000050421368
CreatorName
Hirota, Toru
NRID
1000060321600 1000060321600
researchmap permalink
fukagawa_tatsuo fukagawa_tatsuo
Scopus Author ID
7003912496 7003912496
CreatorName
Fukagawa, Tatsuo
Subject
CCAN
CENP-C
centromere
chromosome segregation
kinetochore assembly
oligomerization
Description
Hara M., Ariyoshi M., Sano T., et al. Centromere/kinetochore is assembled through CENP-C oligomerization. Molecular Cell 83, 2188 (2023); https://doi.org/10.1016/j.molcel.2023.05.023.
Abstract
Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides a platform for the kinetochore assembly. The CCAN protein CENP-C is thought to be a central hub for the centromere/kinetochore organization. However, the role of CENP-C in CCAN assembly needs to be elucidated. Here, we demonstrate that both the CCAN-binding domain and the C-terminal region that includes the Cupin domain of CENP-C are necessary and sufficient for chicken CENP-C function. Structural and biochemical analyses reveal self-oligomerization of the Cupin domains of chicken and human CENP-C. We find that the CENP-C Cupin domain oligomerization is vital for CENP-C function, centromeric localization of CCAN, and centromeric chromatin organization. These results suggest that CENP-C facilitates the centromere/kinetochore assembly through its oligomerization.
Publisher
Cell Press
Source Title
Molecular Cell
Volume (Issue)
83(13)
Page
2188-2205
Date of Issued
2023-07-06
Language
English
Handle URL
https://hdl.handle.net/11094/92473
PISSN
10972765
eISSN
10974164
NCID
AA1119005X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.molcel.2023.05.023
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article