miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis

Iki, Taichiro; Kawaguchi, Shinichi; Kai, Toshie

Science Advances, 2023, 9(29), eadh0397

Number of Access:529（2025-09-05 10:57 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/92526

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Title
miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis
Creator
NRID
1000020774011 1000020774011
ORCID
0000-0002-3215-4552 0000-0002-3215-4552
researchmap permalink
taichiroiki taichiroiki
Scopus Author ID
15049534100 15049534100
CreatorName
Iki, Taichiro
NRID
1000040321749 1000040321749
researchmap permalink
1234567890abc 1234567890abc
Scopus Author ID
36762092600 36762092600
CreatorName
Kawaguchi, Shinichi
NRID
1000040579786 1000040579786
ORCID
0000-0001-8675-8469 0000-0001-8675-8469
researchmap permalink
toshie_kai toshie_kai
Scopus Author ID
7102037941 7102037941
CreatorName
Kai, Toshie
Description
Iki T., Kawaguchi S., Kai T.. miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis. Science Advances 9, eadh0397 (2023); https://doi.org/10.1126/sciadv.adh0397.
Abstract
PIWI-interacting RNA (piRNA) pathways control transposable elements (TEs) and endogenous genes, playing important roles in animal gamete formation. However, the underlying piRNA biogenesis mechanisms remain elusive. Here, we show that endogenous protein coding sequences (CDSs), which are normally used for translation, serve as origins of noncoding piRNA biogenesis in Drosophila melanogaster testes. The product, namely, CDS-piRNAs, formed silencing complexes with Aubergine (Aub) in germ cells. Proximity proteome and functional analyses show that CDS-piRNAs and cluster/TE-piRNAs are distinct species occupying Aub, the former loading selectively relies on chaperone Cyclophilin 40. Moreover, Argonaute 2 (Ago2) and Dicer-2 activities were found critical for CDS-piRNA production. We provide evidence that Ago2-bound short interfering RNAs (siRNAs) and microRNAs (miRNAs) specify precursors to be processed into piRNAs.We further demonstrate that Aub is crucial in spermatid differentiation, regulating chromatins through mRNA cleavage. Collectively, our data illustrate a unique strategy used by male germ line, expanding piRNA repertoire for silencing of endogenous genes during spermatogenesis.
Publisher
American Association for the Advancement of Science
Source Title
Science Advances
Volume (Issue)
9(29)
Page
eadh0397
Date of Issued
2023-07-21
Language
English
Handle URL
https://hdl.handle.net/11094/92526
eISSN
23752548
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1126/sciadv.adh0397
Access Rights
open access
CopyRight
© 2023 The Authors, some rights reserved. This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article