Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats

Mizuno, Shuhei; Matsunaga, Satoru; Kasahara, Norio; Kasahara, Masaaki; Shimoo, Yoshiaki; Abe, Shinichi; Nakano, Takayoshi; Ishimoto, Takuya; Hikita, Atsuhiko; Nojima, Kunihiko; Nishii, Yasushi

doi:https://doi.org/10.3390/jfb14080435

Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats

Mizuno, Shuhei; Matsunaga, Satoru; Kasahara, Norio et al.

Journal of Functional Biomaterials, 2023, 14(8), 435

Number of Access:626（2025-07-18 09:53 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/93109

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Title	Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats
Creator	CreatorName Mizuno, Shuhei
	CreatorName Matsunaga, Satoru
	CreatorName Kasahara, Norio
	NRID 1000060779776 1000060779776 CreatorName Kasahara, Masaaki
	CreatorName Shimoo, Yoshiaki
	NRID 1000040256300 1000040256300 CreatorName Abe, Shinichi
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 CreatorName Ishimoto, Takuya
	NRID 1000060443397 1000060443397 CreatorName Hikita, Atsuhiko
	NRID 1000060189397 1000060189397 CreatorName Nojima, Kunihiko
	CreatorName Nishii, Yasushi
Subject	biological apatite crystallite
	bone quality
	collagen fibre
	microbeam X-ray diffraction
	second harmonic generation imaging
Description	Mizuno S., Matsunaga S., Kasahara N., et al. Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats. Journal of Functional Biomaterials 14, 435 (2023); https://doi.org/10.3390/jfb14080435.
Abstract	The objective of this study is to clarify the effect of restoring the lowered masticatory muscle functional pressure and correcting bilateral differences in masticatory muscle functional pressure on jawbone growth during growth and development with a quantitative evaluation of the changes in the micro/nanostructural characteristics of entheses. Male Wistar rats aged 4 weeks were divided into an experimental group injected with a botulinum toxin serotype A (BoNT/A) formulation to reduce muscle function (BTX group) and a control group (CTRL group). They were euthanised after 6, 8, 10, 12, and 16 weeks after measuring the difference between the midline of the upper and lower incisors. The mandibles were harvested for histological examination, second harmonic generation imaging, and the quantitative evaluation of biological apatite (BAp) crystal alignment. The midline difference decreased with age in weeks. In rats from 6 weeks after BoNT/A administration to 12 weeks after administration, the collagen fibre bundle diameter was significantly smaller in the BTX group; the difference between the two groups decreased with increasing age. BAp crystal alignment was significantly different on the x-axis and the y-axis on the BTX group from 6 weeks after BoNT/A administration to 10 weeks after administration. Asymmetry of mandibular bone formation caused by load imbalance during growth could be corrected by the adjustment of the function of the masseter muscle on either side.
Publisher	Multidisciplinary Digital Publishing Institute (MDPI)
Source Title	Journal of Functional Biomaterials
Volume (Issue)	14(8)
Page	435
Date of Issued	2023-08-21
Language	English
Handle URL	https://hdl.handle.net/11094/93109
eISSN	20794983
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.3390/jfb14080435
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Functional Biomaterials. 2023, 14(8), p. 435
DC.title	Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats
DC.creator	Mizuno, Shuhei
	Matsunaga, Satoru
	Kasahara, Norio
	Kasahara, Masaaki
	Shimoo, Yoshiaki
	Abe, Shinichi
	Nakano, Takayoshi
	Ishimoto, Takuya
	Hikita, Atsuhiko
	Nojima, Kunihiko
	Nishii, Yasushi
DC.publisher	Multidisciplinary Digital Publishing Institute (MDPI)
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-08-21
DC.identifier	https://doi.org/10.3390/jfb14080435
DC.subject	biological apatite crystallite
	bone quality
	collagen fibre
	microbeam X-ray diffraction
	second harmonic generation imaging
DC.description	Mizuno S., Matsunaga S., Kasahara N., et al. Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats. Journal of Functional Biomaterials 14, 435 (2023); https://doi.org/10.3390/jfb14080435.
DCTERMS.abstract	The objective of this study is to clarify the effect of restoring the lowered masticatory muscle functional pressure and correcting bilateral differences in masticatory muscle functional pressure on jawbone growth during growth and development with a quantitative evaluation of the changes in the micro/nanostructural characteristics of entheses. Male Wistar rats aged 4 weeks were divided into an experimental group injected with a botulinum toxin serotype A (BoNT/A) formulation to reduce muscle function (BTX group) and a control group (CTRL group). They were euthanised after 6, 8, 10, 12, and 16 weeks after measuring the difference between the midline of the upper and lower incisors. The mandibles were harvested for histological examination, second harmonic generation imaging, and the quantitative evaluation of biological apatite (BAp) crystal alignment. The midline difference decreased with age in weeks. In rats from 6 weeks after BoNT/A administration to 12 weeks after administration, the collagen fibre bundle diameter was significantly smaller in the BTX group; the difference between the two groups decreased with increasing age. BAp crystal alignment was significantly different on the x-axis and the y-axis on the BTX group from 6 weeks after BoNT/A administration to 10 weeks after administration. Asymmetry of mandibular bone formation caused by load imbalance during growth could be corrected by the adjustment of the function of the masseter muscle on either side.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93109
citation_title	Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats
citation_author	Mizuno, Shuhei
	Matsunaga, Satoru
	Kasahara, Norio
	Kasahara, Masaaki
	Shimoo, Yoshiaki
	Abe, Shinichi
	Nakano, Takayoshi
	Ishimoto, Takuya
	Hikita, Atsuhiko
	Nojima, Kunihiko
	Nishii, Yasushi
citation_publisher	Multidisciplinary Digital Publishing Institute (MDPI)
citation_language	English
citation_date	2023-08-21
citation_journal_title	Journal of Functional Biomaterials
citation_volume	14
citation_issue	8
citation_firstpage	435
citation_doi	https://doi.org/10.3390/jfb14080435
citation_public_url	https://hdl.handle.net/11094/93109

Effect of the Correction of Bilateral Differences in Masseter Muscle Functional Pressure on the Mandible of Growing Rats

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